Cloned (Comment) | Organism |
---|---|
recombinant expression of soluble His-tagged enzyme in Escherichia coli strain ArcticExpress (DE3)RIL | Mytilus galloprovincialis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetazolamide | the recombinant MgaCA shows a very similar acetazolamide inhibition features when compared to those of the native enzyme (MgaCA) | Mytilus galloprovincialis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the recombinant MgaCA shows a very similar kinetic features when compared to those of the native enzyme (MgaCA) | Mytilus galloprovincialis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | the conserved three histidines, His94, His96, and His119 (hCA I numbering system) coordinate the Zn(II) ion that is crucial for catalysis | Mytilus galloprovincialis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2CO3 | Mytilus galloprovincialis | - |
CO2 + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mytilus galloprovincialis | A0A0N9FGL8 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mantle | - |
Mytilus galloprovincialis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2CO3 | - |
Mytilus galloprovincialis | CO2 + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 32000, recombinant enzyme, SDS-PAGE | Mytilus galloprovincialis |
monomer or dimer | x * 50000, native enzyme, SDS-PAGE | Mytilus galloprovincialis |
Synonyms | Comment | Organism |
---|---|---|
alpha-CA | - |
Mytilus galloprovincialis |
alpha-carbonic anhydrase | - |
Mytilus galloprovincialis |
CAII | - |
Mytilus galloprovincialis |
carbonic anhydrase II | - |
Mytilus galloprovincialis |
MgaCA | - |
Mytilus galloprovincialis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Mytilus galloprovincialis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
410000 | - |
CO2 | CO2 hydration, pH 8.3, 22°C, native enzyme | Mytilus galloprovincialis | |
420000 | - |
CO2 | CO2 hydration, pH 8.3, 22°C, recombinant enzyme | Mytilus galloprovincialis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
assay at | Mytilus galloprovincialis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000361 | - |
acetazolamide | CO2 hydration, pH 8.3, 22°C, recombinant enzyme | Mytilus galloprovincialis | |
0.00038 | - |
acetazolamide | CO2 hydration, pH 8.3, 22°C, native enzyme | Mytilus galloprovincialis |
General Information | Comment | Organism |
---|---|---|
additional information | the full nucleotide sequence encoding for the native MgaCA shows an open reading frame of 255 amino acid residues containing the conserved three histidines, His94, His96, and His119 (hCA I numbering system), which coordinate the Zn(II) ion crucial for catalysis, and the two gate-keeper residues, the Glu106 and Thr199. The mussel enzyme has a residue of lysine as substituent of the proton shuttle residue His64, explaining the relatively low catalytic activity of the native MgaCA, with the following kinetic parameters for the CO2 hydration reaction | Mytilus galloprovincialis |
physiological function | in metazoans, the alpha-CA family is largely represented and plays a pivotal role in the deposition of calcium carbonate biominerals | Mytilus galloprovincialis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
35000 | - |
CO2 | CO2 hydration, pH 8.3, 22°C, recombinant enzyme | Mytilus galloprovincialis | |
36000 | - |
CO2 | CO2 hydration, pH 8.3, 22°C, native enzyme | Mytilus galloprovincialis |