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Literature summary for 4.2.1.1 extracted from
- Biochemical characterization of recombinant beta-carbonic anhydrase (PgiCAb) identified in the genome of the oral pathogenic bacterium Porphyromonas gingivalis (2015), J. Enzyme Inhib. Med. Chem., 30, 366-370 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis of PgiCAb, sequence comparisons and phylogenetic tree, recombinant expression of His-tagged enzyme in Escherichia coli | Porphyromonas gingivalis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetazolamide | inhibits the CO2 hydrase activity of PgiCAb | Porphyromonas gingivalis |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | required, metalloenzyme | Porphyromonas gingivalis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2CO3 | Porphyromonas gingivalis | - |
CO2 + H2O | - |
r | |
| H2CO3 | Porphyromonas gingivalis ATCC 33277 | - |
CO2 + H2O | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Porphyromonas gingivalis | B2RL07 | - |
- |
| Porphyromonas gingivalis ATCC 33277 | B2RL07 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CO2 + H2O | - |
Porphyromonas gingivalis | H2CO3 | - |
r | |
| CO2 + H2O | - |
Porphyromonas gingivalis ATCC 33277 | H2CO3 | - |
r | |
| H2CO3 | - |
Porphyromonas gingivalis | CO2 + H2O | - |
r | |
| H2CO3 | - |
Porphyromonas gingivalis ATCC 33277 | CO2 + H2O | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-CA | - |
Porphyromonas gingivalis |
| beta-carbonic anhydrase | - |
Porphyromonas gingivalis |
| PgiCAb | - |
Porphyromonas gingivalis |
| PGJ_00014320 | - |
Porphyromonas gingivalis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at room temperature | Porphyromonas gingivalis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 280000 | - |
CO2 | pH 8.3, 22°C, recombinant His-tagged enzyme | Porphyromonas gingivalis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.3 | - |
assay at | Porphyromonas gingivalis |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000214 | - |
acetazolamide | pH 8.3, 22°C, recombinant His-tagged enzyme | Porphyromonas gingivalis | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 15000 | - |
CO2 | pH 8.3, 22°C, recombinant His-tagged enzyme | Porphyromonas gingivalis | |
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