Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.1.1 extracted from

  • Juozapaitiene, V.; Bartkute, B.; Michailoviene, V.; Zaksauskas, A.; Baranauskiene, L.; Satkune, S.; Matulis, D.
    Purification, enzymatic activity and inhibitor discovery for recombinant human carbonic anhydrase XIV (2016), J. Biotechnol., 240, 31-42 .
    View publication on PubMed
Search for more literature for 4.2.1.1

Application

Application Comment Organism
drug development the isozymes CA IVX is a possible drug target for the treatment of epilepsy, some retinopathies, and skin tumors Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
gene CA14, recombinant expression in Escherichia coli: effect of different promoters, Escherichia coli strains (Rosetta 2(DE3), Rosetta-gami 2 (DE3), Origami B (DE3), and BL21 trxB (DE3)), and the length of recombinant CA XIV protein construct are analyzed for the production CA XIV in large scale by using affinity purification, method evaluation and optimization, overview. The most suitable Escherichia coli strain is Origami B (DE3) Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
2,3,5,6-tetrafluoro-4-(2-phenylethanesulfonyl)benzene-1-sulfonamide
-
 Homo sapiens
2,3,6-trifluoro-4-(2-hydroxyethanesulfonyl)-5-[[(1S)-1,2,3,4-tetrahydronaphthalen-1-yl]amino]benzene-1-sulfonamide
-
 Homo sapiens
3-(benzylamino)-2,5,6-trifluoro-4-(2-hydroxyethanesulfonyl)benzene-1-sulfonamide
-
 Homo sapiens
3-[[(1S)-2,3-dihydro-1H-inden-1-yl]amino]-2,5,6-trifluoro-4-(2-hydroxyethanesulfonyl)benzene-1-sulfonamide
-
 Homo sapiens
3-[[(1S)-2,3-dihydro-1H-inden-1-yl]amino]-2,5,6-trifluoro-4-(2-phenylethanesulfonyl)benzene-1-sulfonamide
-
 Homo sapiens
3-[[(3,4-dimethoxyphenyl)methyl]amino]-2,5,6-trifluoro-4-(2-hydroxyethanesulfonyl)benzene-1-sulfonamide
-
 Homo sapiens
ethoxzolamide strong binding, U-shaped pH-dependence of the Gibbs energy of binding, the affinity is greatest in the near neutral pH region and diminished towards both the acidic and alkaline regions Homo sapiens
additional information 3-[[(3,4-dimethoxyphenyl)methyl]amino]-2,5,6-trifluoro-4-(2-hydroxyethanesulfonyl)benzene-1-sulfonamide does not exhibit selectivity for CA XIV Homo sapiens
trifluoromethanesulfonamide U-shaped pH-dependence of the Gibbs energy of binding, the affinity is greatest in the near neutral pH region and diminished towards both the acidic and alkaline regions Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.9
-
 CO2 pH 7.5, 25°C, recombinant enzyme Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane CA XIV is a membrane-associated glycoprotein Homo sapiens 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ required, metalloenzyme Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CO2 + H2O Homo sapiens
-
 H2CO3
-
 r
H2CO3 Homo sapiens
-
 CO2 + H2O
-
 r

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9ULX7
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein CA XIV is a membrane-associated glycoprotein Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged isozyme CA XIV from Escherichia coli strains to 96% purity by nickel affinity chromatography, dialysis, and 4-aminomethylbenzenesulfonamide affinity chromatography (elution by a linear gradient of 0.5 M sodium perchlorate using 0.1 M sodium acetate and 0-0.5 M sodium perchlorate, pH 5.6) Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
central nervous system
-
 Homo sapiens
-
colon
-
 Homo sapiens
-
eye
-
 Homo sapiens
-
glial cell
-
 Homo sapiens
-
heart
-
 Homo sapiens
-
kidney
-
 Homo sapiens
-
liver
-
 Homo sapiens
-
additional information CA XIV is highly expressed in all parts of the central nervous system and to a lesser extent in adult liver, heart, small intestine, colon, kidney, urinary bladder and skeletal muscle Homo sapiens
-
Mueller cell
-
 Homo sapiens
-
retinal pigment epithelium
-
 Homo sapiens
-
skeletal muscle
-
 Homo sapiens
-
small intestine
-
 Homo sapiens
-
urinary bladder
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CO2 + H2O
-
 Homo sapiens H2CO3
-
 r
H2CO3
-
 Homo sapiens CO2 + H2O
-
 r
additional information reactivity of the purified CA XIV is determined by following the CO2 hydration using the stopped-flow technique Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 37670, recombinant His-tagged enzyme, SDS-PAGE, x * 31998, sequence calculation Homo sapiens
More CA XIV is a membrane-associated glycoprotein composed of an extracellular catalytic domain, a single transmembrane helix, and a short intracellular polypeptide chain. The protein has two cysteine residues that form a disulfide bond for the stabilization of CA XIV structure and one N-glycosylation site Homo sapiens

Synonyms

Synonyms Comment Organism
CA XIV
-
 Homo sapiens
carbonic anhydrase XIV
-
 Homo sapiens
human carbonic anhydrase XIV
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information inhibition kinetics, dissociation constants of compounds 2,3,5,6-tetrafluoro-4-(2-phenylethanesulfonyl)benzene-1-sulfonamide, 3-[[(1S)-2,3-dihydro-1H-inden-1-yl]amino]-2,5,6-trifluoro-4-(2-phenylethanesulfonyl)benzene-1-sulfonamide, 3-(benzylamino)-2,5,6-trifluoro-4-(2-hydroxyethanesulfonyl)benzene-1-sulfonamide, 3-[[(1S)-2,3-dihydro-1H-inden-1-yl]amino]-2,5,6-trifluoro-4-(2-hydroxyethanesulfonyl)benzene-1-sulfonamide and 2,3,6-trifluoro-4-(2-hydroxyethanesulfonyl)-5-[[(1S)-1,2,3,4-tetrahydronaphthalen-1-yl]amino]benzene-1-sulfonamide binding to human recombinant CA XIV at 37°C, sodium phosphate buffer, pH 7.0 Homo sapiens