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Literature summary for 4.2.1.1 extracted from

  • Huyut, Z.; Beydemir, S.; Guelcin, I.
    Inhibition properties of some flavonoids on carbonic anhydrase I and II isoenzymes purified from human erythrocytes (2017), J. Biochem. Mol. Toxicol., 31, e21930 .
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1-(4-methoxyphenyl)-2-methyl-3-nitro-1-H-indol-6-ol ID-8; ID-8 Homo sapiens
callistephin
-
Homo sapiens
malvin
-
Homo sapiens
additional information isozyme hCA II is inhibited by flavonoids, poor inhibition by pelargonin; isozyme hCA I is inhibited by flavonoids, poor inhibition by pelargonin Homo sapiens
oenin
-
Homo sapiens
silychristin
-
Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ required, metalloenzyme Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H2CO3 Homo sapiens
-
CO2 + H2O
-
r

Organism

Organism UniProt Comment Textmining
Homo sapiens P00915
-
-
Homo sapiens P00918
-
-

Purification (Commentary)

Purification (Comment) Organism
native isozyme hCA I from human blood erythrocytes by L-tyrosine-sulfanilamide affinity chromatography and elution with 1.0 M NaCl/25 mM Na2HPO4, pH 6.3 Homo sapiens
native isozyme hCA II from human blood erythrocytes by L-tyrosine-sulfanilamide affinity chromatography and elution with 0.1 M NaCH3COO/0.5 M NaClO4, pH 5.6 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2CO3
-
Homo sapiens CO2 + H2O
-
r
additional information enzyme activity measurements determining CO2-hydratase activity or determining esterase activity with 4-nitrophenyl acetate as substrate Homo sapiens ?
-
?
additional information enzyme activity measurements determining CO2-hydratase activity or determining esterase activity with 4-nitrophenylacetate as substrate Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
CA1
-
Homo sapiens
CA2
-
Homo sapiens
hCA I
-
Homo sapiens
HCA II
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
esterase activity assay at room temperature with 4-nitrophenyl acetate as substrate Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
esterase activity assay at with 4-nitrophenylacetate as substrate Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00035
-
versus CO2 hydration activity, pH and temperature not specified in the publication Homo sapiens oenin
0.0013
-
versus CO2 hydration activity, pH and temperature not specified in the publication Homo sapiens silychristin
0.0187
-
versus CO2 hydration activity, pH and temperature not specified in the publication Homo sapiens oenin
0.0433
-
versus CO2 hydration activity, pH and temperature not specified in the publication Homo sapiens silychristin
0.0462
-
versus CO2 hydration activity, pH and temperature not specified in the publication Homo sapiens callistephin
0.0533
-
versus CO2 hydration activity, pH and temperature not specified in the publication Homo sapiens 1-(4-methoxyphenyl)-2-methyl-3-nitro-1-H-indol-6-ol
0.1155
-
versus CO2 hydration activity, pH and temperature not specified in the publication Homo sapiens malvin
0.231
-
versus CO2 hydration activity, pH and temperature not specified in the publication Homo sapiens malvin
0.3465
-
versus CO2 hydration activity, pH and temperature not specified in the publication Homo sapiens callistephin
0.693
-
versus CO2 hydration activity, pH and temperature not specified in the publication Homo sapiens 1-(4-methoxyphenyl)-2-methyl-3-nitro-1-H-indol-6-ol