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Literature summary for 4.2.1.1 extracted from
- Purification and characterization of the carbonic anhydrase enzyme from Black Sea trout (Salmo trutta Labrax Coruhensis) kidney and inhibition effects of some metal ions on enzyme activity (2016), Environ. Toxicol. Pharmacol., 44, 134-139 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Salmo trutta Labrax Coruhensis |  |
| Co2+ | - |
Salmo trutta Labrax Coruhensis | |
| Cu2+ | - |
Salmo trutta Labrax Coruhensis | |
| Fe2+ | - |
Salmo trutta Labrax Coruhensis | |
| Pb2+ | - |
Salmo trutta Labrax Coruhensis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Lineweaver-Burk kinetic plots | Salmo trutta Labrax Coruhensis | |
| 0.434 | - |
H2CO3 | pH 9.0, 40°C | Salmo trutta Labrax Coruhensis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | a zinc-containing metalloenzyme | Salmo trutta Labrax Coruhensis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2CO3 | Salmo trutta Labrax Coruhensis | - |
CO2 + H2O | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmo trutta Labrax Coruhensis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 349fold by L-tyrosine-sulfanilamide affinity chromatography | Salmo trutta Labrax Coruhensis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Salmo trutta Labrax Coruhensis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the purified enzyme from kidney has a specific activity of 603.77 EU/mg | Salmo trutta Labrax Coruhensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2CO3 | - |
Salmo trutta Labrax Coruhensis | CO2 + H2O | - |
r | |
| additional information | enzyme assay with 4-nitrophenyl acetate as substrate | Salmo trutta Labrax Coruhensis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 29710, SDS-PAGE | Salmo trutta Labrax Coruhensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Salmo trutta Labrax Coruhensis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 688 | - |
H2CO3 | pH 9.0, 40°C | Salmo trutta Labrax Coruhensis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Salmo trutta Labrax Coruhensis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 10 | narrow pH range, profile overview | Salmo trutta Labrax Coruhensis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
stable at | Salmo trutta Labrax Coruhensis |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.185 | - |
Pb2+ | pH 9.0, 40°C | Salmo trutta Labrax Coruhensis | |
| 0.703 | - |
Fe2+ | pH 9.0, 40°C | Salmo trutta Labrax Coruhensis | |
| 1.679 | - |
Co2+ | pH 9.0, 40°C | Salmo trutta Labrax Coruhensis | |
| 18.55 | - |
Ag+ | pH 9.0, 40°C | Salmo trutta Labrax Coruhensis | |
| 48.81 | - |
Cu2+ | pH 9.0, 40°C | Salmo trutta Labrax Coruhensis | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
pH 9.0, 40°C, uncompetitve inhibition | Salmo trutta Labrax Coruhensis | Pb2+ | |
| 0.78 | - |
pH 9.0, 40°C, competitve inhibition | Salmo trutta Labrax Coruhensis | Fe2+ | |
| 2.07 | - |
pH 9.0, 40°C, competitve inhibition | Salmo trutta Labrax Coruhensis | Co2+ | |
| 19.2 | - |
pH 9.0, 40°C, uncompetitve inhibition | Salmo trutta Labrax Coruhensis | Ag+ | |
| 76.7 | - |
pH 9.0, 40°C, competitve inhibition | Salmo trutta Labrax Coruhensis | Cu2+ | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | carbonic anhydrase is a member of zinc-containing metalloenzyme family | Salmo trutta Labrax Coruhensis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1585 | - |
H2CO3 | pH 9.0, 40°C | Salmo trutta Labrax Coruhensis | |
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