Literature summary for 4.2.1.1 extracted from

Langella, E.; Buonanno, M.; Vullo, D.; Dathan, N.; Leone, M.; Supuran, C.T.; De Simone, G.; Monti, S.M.
Biochemical, biophysical and molecular dynamics studies on the proteoglycan-like domain of carbonic anhydrase IX (2018), Cell. Mol. Life Sci., 75, 3283-3296 .

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Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	addition of the PG domain of iszyme CA IX to the catalytic CA-domain increases the catalytic activity by about 63%	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of His-tagged PG domain of isozyme CA9 IX in Escherichia coli strain BL21(DE3)	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	stopped-flow spectrophotometric measurements	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	CA IX is a transmembrane protein, where the extracellular region consists of the catalytic CA domain and the proteoglycan-like (PG) domain	Homo sapiens	16020	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	required	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
50000	-	isolated recombinant detagged PG domain (PG(38-136)), gel filtration, the high retention volume observed is a consequence of a non-globular structure, PG(38-136) has an hydrodynamic dimension typical of IDPs	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
H2CO3	Homo sapiens	-	CO2 + H2O	-	r

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q16790	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged PG domain of isozyme CA9 IX from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage by protease enzyme SenP2, and another step of nickel affinity chromatography to remove the tag	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	human carbonic anhydrase IX (hCA IX) is a tumour-associated enzyme overexpressed in several malignant human tumours	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
H2CO3	-	Homo sapiens	CO2 + H2O	-	r

Subunits

Subunits	Comment	Organism
?	x * 20000, about, isolated recombinant detagged PG domain (PG(38-136)), SDS-PAGE	Homo sapiens
More	the extracellular region of the isozyme consists of the catalytic CA domain and the proteoglycan-like (PG) domain, PG domain structure determination and analysis, overview. The isolated PG domain belongs to the family of intrinsically disordered proteins, being globally unfolded with only some local residual polyproline II secondary structure, it shows conformational flexibility. Primary sequence analysis of the PG(38-136) protein, peptide analysis by gel filtration, NMR spectroscopy, and dynamic light scattering, modelling and molecular dynamics studies, overview	Homo sapiens

Synonyms

Synonyms	Comment	Organism
carbonic anhydrase IX	-	Homo sapiens
hCA IX	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Homo sapiens

General Information

General Information	Comment	Organism
additional information	isozyme modelling and molecular dynamics studies, overview	Homo sapiens
physiological function	human carbonic anhydrase IX (hCA IX) is a tumour-associated enzyme. The observed conformational flexibility of the enzyme's extrcellular PG domain may have several important roles in tumour progression, facilitating interactions of hCA IX with partner proteins assisting tumour spreading and progression	Homo sapiens

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Release 2023.1 (January 2023)