Literature summary for 4.2.1.1 extracted from

De Luca, V.; Del Prete, S.; Carginale, V.; Vullo, D.; Supuran, C.T.; Capasso, C.
Cloning, characterization and anion inhibition studies of a gamma-carbonic anhydrase from the Antarctic cyanobacterium Nostoc commune (2015), Bioorg. Med. Chem. Lett., 25, 4970-4975 .

Cloned(Commentary)

Cloned (Comment)	Organism
DNA and amino acid sequence comparisons, phylogenetic tree of gamma-CAs	Nostoc commune

Inhibitors

Inhibitors	Comment	Organism
acetazolamide	-	Nostoc commune
B4O72-	-	Nostoc commune
Br-	-	Nostoc commune
CF3SO3-	-	Nostoc commune
Cl-	-	Nostoc commune
CN-	-	Nostoc commune
CNO-	-	Nostoc commune
CO32-	-	Nostoc commune
CS32-	-	Nostoc commune
diethyldithiocarbamate	-	Nostoc commune
Et2NCS2-	-	Nostoc commune
FSO3-	-	Nostoc commune
H2NSO2NH2	-	Nostoc commune
H2NSO3H	-	Nostoc commune
HCO3-	-	Nostoc commune
HS-	-	Nostoc commune
HSO3-	-	Nostoc commune
I-	-	Nostoc commune
additional information	fluoride, sulfate, perchlorate and tetrafluoroborate do not inhibit appreciably NcoCA, whereas the other halides, pseudohalides, bicarbonate, nitrate, nitrite and many complex inorganic anions cause inhibition in the millimolar range	Nostoc commune
N3-	-	Nostoc commune
NH(SO3)22-	-	Nostoc commune
NO2-	-	Nostoc commune
NO3-	-	Nostoc commune
P2O74-	-	Nostoc commune
PF6-	-	Nostoc commune
Ph-AsO3H2	-	Nostoc commune
Ph-B(OH)2	-	Nostoc commune
ReO4-	-	Nostoc commune
RuO4-	-	Nostoc commune
S2O82-	-	Nostoc commune
SCN-	-	Nostoc commune
SeCN-	-	Nostoc commune
SeO42-	-	Nostoc commune
SnO32-	-	Nostoc commune
TeO42-	-	Nostoc commune
V2O74-	-	Nostoc commune

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
carboxysome	-	Nostoc commune	31470	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	metal ion ligands are His81, Hist117 and His122	Nostoc commune

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
CO2 + H2O	Nostoc commune	-	H2CO3	-	r
additional information	Nostoc commune	the enzyme has significant catalytic activity for the physiologic reaction, CO2 hydration to bicarbonate and protons	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Nostoc commune	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
CO2 + H2O	-	Nostoc commune	H2CO3	-	r
H2CO3	-	Nostoc commune	CO2 + H2O	-	r
additional information	the enzyme has significant catalytic activity for the physiologic reaction, CO2 hydration to bicarbonate and protons	Nostoc commune	?	-	?
additional information	the enzyme is highly active in the CO2 hydration reaction	Nostoc commune	?	-	?

Synonyms

Synonyms	Comment	Organism
gamma-CA	-	Nostoc commune
gamma-carbonic anhydrase	-	Nostoc commune
NcoCA	-	Nostoc commune

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
950000	-	CO2	pH 7.5, 20°C	Nostoc commune

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism
0.07	-	Ph-B(OH)2	pH 7.5, 20°C, inhibition of CO2 hydration activity	Nostoc commune
0.0758	-	acetazolamide	pH 7.5, 20°C	Nostoc commune
0.081	-	H2NSO2NH2	pH 7.5, 20°C, inhibition of CO2 hydration activity	Nostoc commune
0.084	-	Ph-AsO3H2	pH 7.5, 20°C, inhibition of CO2 hydration activity	Nostoc commune
0.09	-	H2NSO3H	pH 7.5, 20°C, inhibition of CO2 hydration activity	Nostoc commune
0.8	-	diethyldithiocarbamate	pH 7.5, 20°C	Nostoc commune
0.8	-	Et2NCS2-	pH 7.5, 20°C, inhibition of CO2 hydration activity	Nostoc commune
2.4	-	CO32-	pH 7.5, 20°C, inhibition of CO2 hydration activity	Nostoc commune
4.5	-	HCO3-	pH 7.5, 20°C, inhibition of CO2 hydration activity	Nostoc commune
5.4	-	SnO32-	pH 7.5, 20°C, inhibition of CO2 hydration activity	Nostoc commune
6	-	CNO-	pH 7.5, 20°C, inhibition of CO2 hydration activity	Nostoc commune
6.4	-	B4O72-	pH 7.5, 20°C, inhibition of CO2 hydration activity	Nostoc commune
6.7	-	CN-	pH 7.5, 20°C, inhibition of CO2 hydration activity	Nostoc commune
6.9	-	NH(SO3)22-	pH 7.5, 20°C, inhibition of CO2 hydration activity	Nostoc commune
7.3	-	CF3SO3-	pH 7.5, 20°C, inhibition of CO2 hydration activity	Nostoc commune
7.4	-	PF6-	pH 7.5, 20°C, inhibition of CO2 hydration activity	Nostoc commune
7.6	-	V2O74-	pH 7.5, 20°C, inhibition of CO2 hydration activity	Nostoc commune
7.6	-	RuO4-	pH 7.5, 20°C, inhibition of CO2 hydration activity	Nostoc commune
7.7	-	TeO42-	pH 7.5, 20°C, inhibition of CO2 hydration activity	Nostoc commune
8	-	SeCN-	pH 7.5, 20°C, inhibition of CO2 hydration activity	Nostoc commune
8.1	-	SCN-	pH 7.5, 20°C, inhibition of CO2 hydration activity	Nostoc commune
8.5	-	SeO42-	pH 7.5, 20°C, inhibition of CO2 hydration activity	Nostoc commune
8.7	-	Cl-	pH 7.5, 20°C, inhibition of CO2 hydration activity	Nostoc commune
8.8	-	NO2-	pH 7.5, 20°C, inhibition of CO2 hydration activity	Nostoc commune
8.8	-	ReO4-	pH 7.5, 20°C, inhibition of CO2 hydration activity	Nostoc commune
8.9	-	I-	pH 7.5, 20°C, inhibition of CO2 hydration activity	Nostoc commune
8.9	-	CS32-	pH 7.5, 20°C, inhibition of CO2 hydration activity	Nostoc commune
9.1	-	P2O74-	pH 7.5, 20°C, inhibition of CO2 hydration activity	Nostoc commune
9.3	-	Br-	pH 7.5, 20°C, inhibition of CO2 hydration activity	Nostoc commune
9.5	-	FSO3-	pH 7.5, 20°C, inhibition of CO2 hydration activity	Nostoc commune
9.5	-	S2O82-	pH 7.5, 20°C, inhibition of CO2 hydration activity	Nostoc commune
14.9	-	HSO3-	pH 7.5, 20°C, inhibition of CO2 hydration activity	Nostoc commune
20.4	-	HS-	pH 7.5, 20°C, inhibition of CO2 hydration activity	Nostoc commune
27.6	-	NO3-	pH 7.5, 20°C, inhibition of CO2 hydration activity	Nostoc commune
30.5	-	N3-	pH 7.5, 20°C, inhibition of CO2 hydration activity	Nostoc commune

General Information

General Information	Comment	Organism
evolution	phylogenetic tree of gamma-CAs	Nostoc commune
physiological function	gamma-CAs are present in carboxysomes, being involved in photosynthesis	Nostoc commune

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
83000	-	CO2	pH 7.5, 20°C	Nostoc commune