Cloned (Comment) | Organism |
---|---|
gene PhaCAgamma, sequence comparisons and phylogenetic tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)(ArcticExpress DE3 competent cells), subcloning in Escherichia coli strain DH5alpha | Pseudoalteromonas haloplanktis ANT/505 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetazolamide | sulfonamide CA inhibitor (CAI) | Pseudoalteromonas haloplanktis ANT/505 | |
B4O72- | - |
Pseudoalteromonas haloplanktis ANT/505 | |
Br- | - |
Pseudoalteromonas haloplanktis ANT/505 | |
CN- | - |
Pseudoalteromonas haloplanktis ANT/505 | |
CNO- | - |
Pseudoalteromonas haloplanktis ANT/505 | |
CO32- | - |
Pseudoalteromonas haloplanktis ANT/505 | |
CS32- | - |
Pseudoalteromonas haloplanktis ANT/505 | |
Et2NCS2- | - |
Pseudoalteromonas haloplanktis ANT/505 | |
FSO3- | - |
Pseudoalteromonas haloplanktis ANT/505 | |
H2NSO2NH2 | - |
Pseudoalteromonas haloplanktis ANT/505 | |
H2NSO3H | - |
Pseudoalteromonas haloplanktis ANT/505 | |
HCO3- | - |
Pseudoalteromonas haloplanktis ANT/505 | |
HS- | - |
Pseudoalteromonas haloplanktis ANT/505 | |
HSO3- | - |
Pseudoalteromonas haloplanktis ANT/505 | |
additional information | no inhibition by fluoride, iodide, chloride, azide, sulfate, P2O74-, V2O74-, ReO4-, chlorate, tetrafluoroborate, PF6-, and CF3SO3- | Pseudoalteromonas haloplanktis ANT/505 | |
NH(SO3)22- | - |
Pseudoalteromonas haloplanktis ANT/505 | |
NO2- | - |
Pseudoalteromonas haloplanktis ANT/505 | |
NO3- | - |
Pseudoalteromonas haloplanktis ANT/505 | |
Ph-AsO3H2 | - |
Pseudoalteromonas haloplanktis ANT/505 | |
Ph-B(OH)2 | - |
Pseudoalteromonas haloplanktis ANT/505 | |
RuO4- | - |
Pseudoalteromonas haloplanktis ANT/505 | |
S2O82- | - |
Pseudoalteromonas haloplanktis ANT/505 | |
SCN- | - |
Pseudoalteromonas haloplanktis ANT/505 | |
SeCN- | - |
Pseudoalteromonas haloplanktis ANT/505 | |
SeO42- | - |
Pseudoalteromonas haloplanktis ANT/505 | |
SnO32- | - |
Pseudoalteromonas haloplanktis ANT/505 | |
TeO42- | - |
Pseudoalteromonas haloplanktis ANT/505 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO2 + H2O | Pseudoalteromonas haloplanktis ANT/505 | - |
H2CO3 | - |
r | |
H2CO3 | Pseudoalteromonas haloplanktis ANT/505 | - |
CO2 + H2O | - |
r | |
additional information | Pseudoalteromonas haloplanktis ANT/505 | the enzyme has a medium-low catalytic activity for the physiologic reaction of CO2 hydration to bicarbonate and protons | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudoalteromonas haloplanktis ANT/505 | F3BJI2 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)/ArcticExpress DE3 cells by nickel affinity chromatography | Pseudoalteromonas haloplanktis ANT/505 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO2 + H2O | - |
Pseudoalteromonas haloplanktis ANT/505 | H2CO3 | - |
r | |
H2CO3 | - |
Pseudoalteromonas haloplanktis ANT/505 | CO2 + H2O | - |
r | |
additional information | the enzyme has a medium-low catalytic activity for the physiologic reaction of CO2 hydration to bicarbonate and protons | Pseudoalteromonas haloplanktis ANT/505 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
gamma-carbonic anhydrase | - |
Pseudoalteromonas haloplanktis ANT/505 |
PhaCAgamma | - |
Pseudoalteromonas haloplanktis ANT/505 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Pseudoalteromonas haloplanktis ANT/505 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
140000 | - |
CO2 | pH 8.3, 20°C | Pseudoalteromonas haloplanktis ANT/505 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
assay at | Pseudoalteromonas haloplanktis ANT/505 |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000403 | - |
acetazolamide | pH 8.3, 20°C | Pseudoalteromonas haloplanktis ANT/505 | |
0.082 | - |
H2NSO2NH2 | pH 8.3, 20°C, inhibition of CO2 hydration activity | Pseudoalteromonas haloplanktis ANT/505 | |
0.087 | - |
Ph-AsO3H2 | pH 8.3, 20°C, inhibition of CO2 hydration activity | Pseudoalteromonas haloplanktis ANT/505 | |
0.088 | - |
Ph-B(OH)2 | pH 8.3, 20°C, inhibition of CO2 hydration activity | Pseudoalteromonas haloplanktis ANT/505 | |
0.091 | - |
H2NSO3H | pH 8.3, 20°C, inhibition of CO2 hydration activity | Pseudoalteromonas haloplanktis ANT/505 | |
0.96 | - |
Et2NCS2- | pH 8.3, 20°C, inhibition of CO2 hydration activity | Pseudoalteromonas haloplanktis ANT/505 | |
1.7 | - |
CNO- | pH 8.3, 20°C, inhibition of CO2 hydration activity | Pseudoalteromonas haloplanktis ANT/505 | |
2.5 | - |
CO32- | pH 8.3, 20°C, inhibition of CO2 hydration activity | Pseudoalteromonas haloplanktis ANT/505 | |
5.6 | - |
NO3- | pH 8.3, 20°C, inhibition of CO2 hydration activity | Pseudoalteromonas haloplanktis ANT/505 | |
5.9 | - |
CN- | pH 8.3, 20°C, inhibition of CO2 hydration activity | Pseudoalteromonas haloplanktis ANT/505 | |
6.1 | - |
SeO42- | pH 8.3, 20°C, inhibition of CO2 hydration activity | Pseudoalteromonas haloplanktis ANT/505 | |
6.2 | - |
HCO3- | pH 8.3, 20°C, inhibition of CO2 hydration activity | Pseudoalteromonas haloplanktis ANT/505 | |
6.5 | - |
NH(SO3)22- | pH 8.3, 20°C, inhibition of CO2 hydration activity | Pseudoalteromonas haloplanktis ANT/505 | |
6.7 | - |
NO2- | pH 8.3, 20°C, inhibition of CO2 hydration activity | Pseudoalteromonas haloplanktis ANT/505 | |
6.7 | - |
SnO32- | pH 8.3, 20°C, inhibition of CO2 hydration activity | Pseudoalteromonas haloplanktis ANT/505 | |
7.7 | - |
FSO3- | pH 8.3, 20°C, inhibition of CO2 hydration activity | Pseudoalteromonas haloplanktis ANT/505 | |
7.8 | - |
SeCN- | pH 8.3, 20°C, inhibition of CO2 hydration activity | Pseudoalteromonas haloplanktis ANT/505 | |
8 | - |
B4O72- | pH 8.3, 20°C, inhibition of CO2 hydration activity | Pseudoalteromonas haloplanktis ANT/505 | |
8.4 | - |
HS- | pH 8.3, 20°C, inhibition of CO2 hydration activity | Pseudoalteromonas haloplanktis ANT/505 | |
8.5 | - |
TeO42- | pH 8.3, 20°C, inhibition of CO2 hydration activity | Pseudoalteromonas haloplanktis ANT/505 | |
8.7 | - |
Br- | pH 8.3, 20°C, inhibition of CO2 hydration activity | Pseudoalteromonas haloplanktis ANT/505 | |
8.7 | - |
RuO4- | pH 8.3, 20°C, inhibition of CO2 hydration activity | Pseudoalteromonas haloplanktis ANT/505 | |
9.1 | - |
S2O82- | pH 8.3, 20°C, inhibition of CO2 hydration activity | Pseudoalteromonas haloplanktis ANT/505 | |
9.3 | - |
SCN- | pH 8.3, 20°C, inhibition of CO2 hydration activity | Pseudoalteromonas haloplanktis ANT/505 | |
24.1 | - |
HSO3- | pH 8.3, 20°C, inhibition of CO2 hydration activity | Pseudoalteromonas haloplanktis ANT/505 | |
34.6 | - |
CS32- | pH 8.3, 20°C, inhibition of CO2 hydration activity | Pseudoalteromonas haloplanktis ANT/505 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1900 | - |
CO2 | pH 8.3, 20°C | Pseudoalteromonas haloplanktis ANT/505 |