Literature summary for 4.2.1.1 extracted from

Pan, P.; Vermelho, A.B.; Capaci Rodrigues, G.; Scozzafava, A.; Tolvanen, M.E.; Parkkila, S.; Capasso, C.; Supuran, C.T.
Cloning, characterization, and sulfonamide and thiol inhibition studies of an alpha-carbonic anhydrase from Trypanosoma cruzi, the causative agent of Chagas disease (2013), J. Med. Chem., 56, 1761-1771.

Cloned(Commentary)

Cloned (Comment)	Organism
in Escherichia coli as a GST-fusion protein	Malassezia globosa
using the baculovirus expression system as a His-tagged fusion protein	Trypanosoma cruzi

Inhibitors

Inhibitors	Comment	Organism
(2Z)-2-(2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide	-	Trypanosoma cruzi
(2Z)-2-(3-bromobenzylidene)-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide	-	Trypanosoma cruzi
(2Z)-2-(3-methoxybenzylidene)-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide	-	Trypanosoma cruzi
(2Z)-2-(4-chlorobenzylidene)-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide	-	Trypanosoma cruzi
(2Z)-2-(4-methylpentan-2-ylidene)-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide	-	Trypanosoma cruzi
(2Z)-2-(5-bromo-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide	-	Trypanosoma cruzi
(2Z)-2-benzylidene-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide	-	Trypanosoma cruzi
(4R)-4-(ethylamino)-2-(3-methoxypropyl)-3,4-dihydro-2H-thieno[3,2-e][1,2]thiazine-6-sulfonamide 1,1-dioxide	-	Malassezia globosa
(4R)-4-(ethylamino)-2-(3-methoxypropyl)-3,4-dihydro-2H-thieno[3,2-e][1,2]thiazine-6-sulfonamide 1,1-dioxide	-	Trypanosoma cruzi
(4S,6S)-4-(ethylamino)-6-methyl-5,6-dihydro-4H-thieno[2,3-b]thiopyran-2-sulfonamide 7,7-dioxide	-	Malassezia globosa
(4S,6S)-4-(ethylamino)-6-methyl-5,6-dihydro-4H-thieno[2,3-b]thiopyran-2-sulfonamide 7,7-dioxide	-	Trypanosoma cruzi
1,2-benzothiazol-3(2H)-one 1,1-dioxide	-	Trypanosoma cruzi
1-(1,2-benzoxazol-3-yl)methanesulfonamide	-	Malassezia globosa
1-(1,2-benzoxazol-3-yl)methanesulfonamide	-	Trypanosoma cruzi
2-(hydroxymethyl)-4-nitro-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide	-	Trypanosoma cruzi
2-aminobenzenesulfonamide	-	Malassezia globosa
2-methoxy-N-[(1-methylpyrrolidin-2-yl)methyl]-5-sulfamoylbenzamide	-	Malassezia globosa
2-methoxy-N-[(1-methylpyrrolidin-2-yl)methyl]-5-sulfamoylbenzamide	-	Trypanosoma cruzi
3,5-dichloro-2-hydroxybenzenesulfonamide	-	Trypanosoma cruzi
3-(4-sulfamoylphenyl)propanoic acid	-	Malassezia globosa
3-aminobenzenesulfonamide	-	Trypanosoma cruzi
4,5-dichlorobenzene-1,3-disulfonamide	-	Malassezia globosa
4,5-dichlorobenzene-1,3-disulfonamide	-	Trypanosoma cruzi
4-(1,1-dioxido-1,2-thiazinan-2-yl)benzenesulfonamide	-	Trypanosoma cruzi
4-(2-aminoethyl)benzenesulfonamide	-	Malassezia globosa
4-(2-aminoethyl)benzenesulfonamide	-	Trypanosoma cruzi
4-(2-hydroxyethyl)benzenesulfonamide	-	Malassezia globosa
4-(2-hydroxyethyl)benzenesulfonamide	-	Trypanosoma cruzi
4-(5-methyl-3-phenyl-1,2-oxazol-4-yl)benzenesulfonamide	-	Malassezia globosa
4-(5-methyl-3-phenyl-1,2-oxazol-4-yl)benzenesulfonamide	-	Trypanosoma cruzi
4-(aminomethyl)benzenesulfonamide	-	Malassezia globosa
4-(aminomethyl)benzenesulfonamide	-	Trypanosoma cruzi
4-(hydroxymethyl)benzenesulfonamide	-	Malassezia globosa
4-(hydroxymethyl)benzenesulfonamide	-	Trypanosoma cruzi
4-amino-3,bromobenzenesulfonamide	-	Trypanosoma cruzi
4-amino-3-bromobenzenesulfonamide	-	Malassezia globosa
4-amino-3-chlorobenzenesulfonamide	-	Malassezia globosa
4-amino-3-chlorobenzenesulfonamide	-	Trypanosoma cruzi
4-amino-3-fluorobenzenesulfonamide	-	Malassezia globosa
4-amino-3-fluorobenzenesulfonamide	-	Trypanosoma cruzi
4-amino-3-iodobenzenesulfonamide	-	Malassezia globosa
4-amino-6-(trifluoromethyl)benzene-1,3-disulfonamide	-	Malassezia globosa
4-amino-6-(trifluoromethyl)benzene-1,3-disulfonamide	-	Trypanosoma cruzi
4-amino-6-chlorobenzene-1,3-disulfonamide	-	Malassezia globosa
4-amino-6-chlorobenzene-1,3-disulfonamide	-	Trypanosoma cruzi
4-amino-N-(4-sulfamoylbenzyl)benzenesulfonamide	-	Malassezia globosa
4-amino-N-(4-sulfamoylbenzyl)benzenesulfonamide	-	Trypanosoma cruzi
4-amino-N-(4-sulfamoylphenyl)benzenesulfonamide	-	Malassezia globosa
4-amino-N-(4-sulfamoylphenyl)benzenesulfonamide	-	Trypanosoma cruzi
4-amino-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide	-	Malassezia globosa
4-amino-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide	-	Trypanosoma cruzi
4-Aminobenzenesulfonamide	-	Malassezia globosa
4-Aminobenzenesulfonamide	-	Trypanosoma cruzi
4-bromobenzenesulfonamide	-	Malassezia globosa
4-bromophenyl sulfamate	-	Malassezia globosa
4-hydroxybenzenesulfonamide	-	Trypanosoma cruzi
4-sulfamoylbenzoic acid	-	Malassezia globosa
4-sulfamoylbenzoic acid	-	Trypanosoma cruzi
4-[(2-aminopyrimidin-4-yl)amino]benzenesulfonamide	-	Malassezia globosa
4-[(2-aminopyrimidin-4-yl)amino]benzenesulfonamide	-	Trypanosoma cruzi
4-[5-(4-methylphenyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl]benzenesulfonamide	-	Malassezia globosa
4-[5-(4-methylphenyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl]benzenesulfonamide	-	Trypanosoma cruzi
5-(3-chlorophenyl)-1,3,4-thiadiazole-2-sulfonamide	-	Malassezia globosa
5-([(4-aminophenyl)sulfonyl]amino)-1,3,4-thiadiazole-2-sulfonamide	-	Trypanosoma cruzi
5-amino-1,3,4-thiadiazole-2-sulfonamide	-	Malassezia globosa
5-amino-1,3,4-thiadiazole-2-sulfonamide	-	Trypanosoma cruzi
5-imino-4-methyl-4,5-dihydro-1,3,4-thiadiazole-2-sulfonamide	-	Malassezia globosa
5-imino-4-methyl-4,5-dihydro-1,3,4-thiadiazole-2-sulfonamide	-	Trypanosoma cruzi
5-[(phenylsulfonyl)amino]-1,3,4-thiadiazole-2-sulfonamide	-	Malassezia globosa
5-[(phenylsulfonyl)amino]-1,3,4-thiadiazole-2-sulfonamide	-	Trypanosoma cruzi
6-chloro-3,4-dihydro-2H-1,2,4-benzothiadiazine-7-sulfonamide 1,1-dioxide	-	Trypanosoma cruzi
6-ethoxy-1,3-benzothiazole-2-sulfonamide	-	Malassezia globosa
6-ethoxy-1,3-benzothiazole-2-sulfonamide	-	Trypanosoma cruzi
acetazolamide	-	Trypanosoma cruzi
benzene-1,3-disulfonamide	-	Trypanosoma cruzi
benzenesulfonamide	-	Malassezia globosa
benzenesulfonamide	-	Trypanosoma cruzi
N-(3-chloro-1H-indol-7-yl)benzene-1,4-disulfonamide	-	Malassezia globosa
N-(3-chloro-1H-indol-7-yl)benzene-1,4-disulfonamide	-	Trypanosoma cruzi
N-(4-bromophenyl)sulfuric diamide	-	Malassezia globosa
N-(5-sulfamoyl-1,3,4-thiadiazol-2-yl)acetamide	-	Malassezia globosa
N-(5-sulfamoyl-1,3,4-thiadiazol-2-yl)acetamide	-	Trypanosoma cruzi
N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide	-	Trypanosoma cruzi
N-phenylsulfuric diamide	-	Malassezia globosa
N-[(2E)-3-methyl-5-sulfamoyl-1,3,4-thiadiazol-2(3H)-ylidene]acetamide	-	Malassezia globosa
N-[(2E)-3-methyl-5-sulfamoyl-1,3,4-thiadiazol-2(3H)-ylidene]acetamide	-	Trypanosoma cruzi
phenyl sulfamate	-	Malassezia globosa
[(3aS,5aR,8aR,8bS)-2,2,7,7-tetramethyltetrahydro-3aH-bis[1,3]dioxolo[4,5-b:4',5'-d]pyran-3a-yl]methyl sulfamate	-	Malassezia globosa
[(3aS,5aR,8aR,8bS)-2,2,7,7-tetramethyltetrahydro-3aH-bis[1,3]dioxolo[4,5-b:4',5'-d]pyran-3a-yl]methyl sulfamate	-	Trypanosoma cruzi

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
8.1	-	CO2	pH 7.5, 20°C	Trypanosoma cruzi
8.1	-	CO2	pH 8.3, 20°C	Malassezia globosa

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
34000	-	SDS-PAGE	Trypanosoma cruzi
34000	-	SDS-PAGE	Malassezia globosa

Organism

Organism	UniProt	Comment	Textmining
Malassezia globosa	-	-	-
Trypanosoma cruzi	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
CO2 + H2O	-	Trypanosoma cruzi	H2CO3	-	?
CO2 + H2O	-	Malassezia globosa	H2CO3	-	?

Synonyms

Synonyms	Comment	Organism
alpha-carbonic anhydrase	-	Trypanosoma cruzi
beta-carbonic anhydrase	-	Malassezia globosa
MG-CA	-	Malassezia globosa
TcCA	-	Trypanosoma cruzi

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
860000	-	CO2	pH 8.3, 20°C	Malassezia globosa
1210000	-	CO2	pH 7.5, 20°C	Trypanosoma cruzi

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism
0.0000211	-	(2Z)-2-benzylidene-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide	pH 7.5, 20°C	Trypanosoma cruzi
0.0000345	-	(2Z)-2-(3-methoxybenzylidene)-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide	pH 7.5, 20°C	Trypanosoma cruzi
0.0000431	-	(2Z)-2-(3-bromobenzylidene)-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide	pH 7.5, 20°C	Trypanosoma cruzi
0.0000524	-	(2Z)-2-(4-methylpentan-2-ylidene)-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide	pH 7.5, 20°C	Trypanosoma cruzi
0.0000616	-	acetazolamide	pH 7.5, 20°C	Trypanosoma cruzi
0.0000616	-	N-(5-sulfamoyl-1,3,4-thiadiazol-2-yl)acetamide	pH 7.5, 20°C	Trypanosoma cruzi
0.000063	-	4-amino-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide	pH 8.3, 20°C	Malassezia globosa
0.0000643	-	(2Z)-2-(4-chlorobenzylidene)-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide	pH 7.5, 20°C	Trypanosoma cruzi
0.000068	-	4-[(2-aminopyrimidin-4-yl)amino]benzenesulfonamide	pH 8.3, 20°C	Malassezia globosa
0.0000719	-	4-(1,1-dioxido-1,2-thiazinan-2-yl)benzenesulfonamide	pH 7.5, 20°C	Trypanosoma cruzi
0.0000725	-	(2Z)-2-(5-bromo-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide	pH 7.5, 20°C	Trypanosoma cruzi
0.0000749	-	N-[(2E)-3-methyl-5-sulfamoyl-1,3,4-thiadiazol-2(3H)-ylidene]acetamide	pH 7.5, 20°C	Trypanosoma cruzi
0.000079	-	(2Z)-2-(2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide	pH 7.5, 20°C	Trypanosoma cruzi
0.000079	-	4-(2-aminoethyl)benzenesulfonamide	pH 8.3, 20°C	Malassezia globosa
0.0000827	-	4-[5-(4-methylphenyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl]benzenesulfonamide	pH 7.5, 20°C	Trypanosoma cruzi
0.0000845	-	N-(3-chloro-1H-indol-7-yl)benzene-1,4-disulfonamide	pH 7.5, 20°C	Trypanosoma cruzi
0.0000855	-	[(3aS,5aR,8aR,8bS)-2,2,7,7-tetramethyltetrahydro-3aH-bis[1,3]dioxolo[4,5-b:4',5'-d]pyran-3a-yl]methyl sulfamate	pH 7.5, 20°C	Trypanosoma cruzi
0.0000873	-	(4R)-4-(ethylamino)-2-(3-methoxypropyl)-3,4-dihydro-2H-thieno[3,2-e][1,2]thiazine-6-sulfonamide 1,1-dioxide	pH 7.5, 20°C	Trypanosoma cruzi
0.0000879	-	2-methoxy-N-[(1-methylpyrrolidin-2-yl)methyl]-5-sulfamoylbenzamide	pH 7.5, 20°C	Trypanosoma cruzi
0.0000882	-	6-ethoxy-1,3-benzothiazole-2-sulfonamide	pH 7.5, 20°C	Trypanosoma cruzi
0.0000885	-	5-([(4-aminophenyl)sulfonyl]amino)-1,3,4-thiadiazole-2-sulfonamide	pH 7.5, 20°C	Trypanosoma cruzi
0.00009	-	4-amino-6-(trifluoromethyl)benzene-1,3-disulfonamide	pH 8.3, 20°C	Malassezia globosa
0.0000911	-	4-(5-methyl-3-phenyl-1,2-oxazol-4-yl)benzenesulfonamide	pH 7.5, 20°C	Trypanosoma cruzi
0.000092	-	4-amino-6-chlorobenzene-1,3-disulfonamide	pH 8.3, 20°C	Malassezia globosa
0.0000929	-	(4S,6S)-4-(ethylamino)-6-methyl-5,6-dihydro-4H-thieno[2,3-b]thiopyran-2-sulfonamide 7,7-dioxide	pH 7.5, 20°C	Trypanosoma cruzi
0.0000936	-	5-[(phenylsulfonyl)amino]-1,3,4-thiadiazole-2-sulfonamide	pH 7.5, 20°C	Trypanosoma cruzi
0.000094	-	4-sulfamoylbenzoic acid	pH 8.3, 20°C	Malassezia globosa
0.0000947	-	(2Z)-2-(4-chlorobenzylidene)-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide	pH 7.5, 20°C	Trypanosoma cruzi
0.000104	-	4-amino-N-(4-sulfamoylbenzyl)benzenesulfonamide	pH 8.3, 20°C	Malassezia globosa
0.000113	-	N-(3-chloro-1H-indol-7-yl)benzene-1,4-disulfonamide	pH 8.3, 20°C	Malassezia globosa
0.000116	-	4-amino-3-fluorobenzenesulfonamide	pH 8.3, 20°C	Malassezia globosa
0.000118	-	4-(2-hydroxyethyl)benzenesulfonamide	pH 8.3, 20°C	Malassezia globosa
0.000121	-	4-amino-3-chlorobenzenesulfonamide	pH 8.3, 20°C	Malassezia globosa
0.000125	-	N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide	pH 7.5, 20°C	Trypanosoma cruzi
0.000128	-	4,5-dichlorobenzene-1,3-disulfonamide	pH 7.5, 20°C	Trypanosoma cruzi
0.000134	-	6-chloro-3,4-dihydro-2H-1,2,4-benzothiadiazine-7-sulfonamide 1,1-dioxide	pH 7.5, 20°C	Trypanosoma cruzi
0.000134	-	2-(hydroxymethyl)-4-nitro-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide	pH 7.5, 20°C	Trypanosoma cruzi
0.000152	-	3-(4-sulfamoylphenyl)propanoic acid	pH 8.3, 20°C	Malassezia globosa
0.000174	-	4-(aminomethyl)benzenesulfonamide	pH 8.3, 20°C	Malassezia globosa
0.000192	-	4-amino-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide	pH 7.5, 20°C	Trypanosoma cruzi
0.000234	-	4-(hydroxymethyl)benzenesulfonamide	pH 8.3, 20°C	Malassezia globosa
0.000236	-	4-amino-N-(4-sulfamoylphenyl)benzenesulfonamide	pH 8.3, 20°C	Malassezia globosa
0.000243	-	4-amino-N-(4-sulfamoylbenzyl)benzenesulfonamide	pH 7.5, 20°C	Trypanosoma cruzi
0.000245	-	4-Aminobenzenesulfonamide	pH 8.3, 20°C	Malassezia globosa
0.00032	-	2-methoxy-N-[(1-methylpyrrolidin-2-yl)methyl]-5-sulfamoylbenzamide	pH 8.3, 20°C	Malassezia globosa
0.000346	-	4,5-dichlorobenzene-1,3-disulfonamide	pH 8.3, 20°C	Malassezia globosa
0.000349	-	4-amino-3-bromobenzenesulfonamide	pH 8.3, 20°C	Malassezia globosa
0.000365	-	4-amino-N-(4-sulfamoylphenyl)benzenesulfonamide	pH 7.5, 20°C	Trypanosoma cruzi
0.000374	-	4-bromophenyl sulfamate	pH 8.3, 20°C	Malassezia globosa
0.000413	-	N-(4-bromophenyl)sulfuric diamide	pH 8.3, 20°C	Malassezia globosa
0.000482	-	5-[(phenylsulfonyl)amino]-1,3,4-thiadiazole-2-sulfonamide	pH 8.3, 20°C	Malassezia globosa
0.000534	-	5-amino-1,3,4-thiadiazole-2-sulfonamide	pH 7.5, 20°C	Trypanosoma cruzi
0.000543	-	4-amino-3-iodobenzenesulfonamide	pH 8.3, 20°C	Malassezia globosa
0.00065	-	4-bromobenzenesulfonamide	pH 8.3, 20°C	Malassezia globosa
0.000652	-	5-imino-4-methyl-4,5-dihydro-1,3,4-thiadiazole-2-sulfonamide	pH 7.5, 20°C	Trypanosoma cruzi
0.00081	-	4-[(2-aminopyrimidin-4-yl)amino]benzenesulfonamide	pH 7.5, 20°C	Trypanosoma cruzi
0.00082	-	4-amino-6-chlorobenzene-1,3-disulfonamide	pH 7.5, 20°C	Trypanosoma cruzi
0.000842	-	4-amino-6-(trifluoromethyl)benzene-1,3-disulfonamide	pH 7.5, 20°C	Trypanosoma cruzi
0.000867	-	1-(1,2-benzoxazol-3-yl)methanesulfonamide	pH 7.5, 20°C	Trypanosoma cruzi
0.00146	-	[(3aS,5aR,8aR,8bS)-2,2,7,7-tetramethyltetrahydro-3aH-bis[1,3]dioxolo[4,5-b:4',5'-d]pyran-3a-yl]methyl sulfamate	pH 8.3, 20°C	Malassezia globosa
0.00256	-	phenyl sulfamate	pH 8.3, 20°C	Malassezia globosa
0.0031	-	N-phenylsulfuric diamide	pH 8.3, 20°C	Malassezia globosa
0.00453	-	benzenesulfonamide	pH 8.3, 20°C	Malassezia globosa
0.00674	-	benzenesulfonamide	pH 8.3, 20°C	Malassezia globosa
0.006925	-	4-amino-3-chlorobenzenesulfonamide	pH 7.5, 20°C	Trypanosoma cruzi
0.007231	-	4-(aminomethyl)benzenesulfonamide	pH 7.5, 20°C	Trypanosoma cruzi
0.00765	-	1-(1,2-benzoxazol-3-yl)methanesulfonamide	pH 8.3, 20°C	Malassezia globosa
0.00813	-	4-amino-3-fluorobenzenesulfonamide	pH 7.5, 20°C	Trypanosoma cruzi
0.00821	-	1,2-benzothiazol-3(2H)-one 1,1-dioxide	pH 7.5, 20°C	Trypanosoma cruzi
0.00852	-	4-amino-3,bromobenzenesulfonamide	pH 7.5, 20°C	Trypanosoma cruzi
0.009238	-	4-(2-aminoethyl)benzenesulfonamide	pH 7.5, 20°C	Trypanosoma cruzi
0.009433	-	3,5-dichloro-2-hydroxybenzenesulfonamide	pH 7.5, 20°C	Trypanosoma cruzi
0.0098	-	2-aminobenzenesulfonamide	pH 8.3, 20°C	Malassezia globosa
0.02546	-	3-aminobenzenesulfonamide	pH 7.5, 20°C	Trypanosoma cruzi
0.0315	-	4-(5-methyl-3-phenyl-1,2-oxazol-4-yl)benzenesulfonamide	pH 8.3, 20°C	Malassezia globosa
0.0348	-	4-[5-(4-methylphenyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl]benzenesulfonamide	pH 8.3, 20°C	Malassezia globosa
0.035	-	5-(3-chlorophenyl)-1,3,4-thiadiazole-2-sulfonamide	pH 8.3, 20°C	Malassezia globosa
0.038	-	6-ethoxy-1,3-benzothiazole-2-sulfonamide	pH 8.3, 20°C	Malassezia globosa
0.0442	-	benzenesulfonamide	pH 7.5, 20°C	Trypanosoma cruzi
0.0573	-	4-Aminobenzenesulfonamide	pH 7.5, 20°C	Trypanosoma cruzi
0.0638	-	benzene-1,3-disulfonamide	pH 7.5, 20°C	Trypanosoma cruzi
0.06675	-	4-(2-hydroxyethyl)benzenesulfonamide	pH 7.5, 20°C	Trypanosoma cruzi
0.07185	-	4-(hydroxymethyl)benzenesulfonamide	pH 7.5, 20°C	Trypanosoma cruzi
0.07388	-	4-hydroxybenzenesulfonamide	pH 7.5, 20°C	Trypanosoma cruzi
0.07455	-	N-[(2E)-3-methyl-5-sulfamoyl-1,3,4-thiadiazol-2(3H)-ylidene]acetamide	pH 8.3, 20°C	Malassezia globosa
0.076	-	N-(5-sulfamoyl-1,3,4-thiadiazol-2-yl)acetamide	pH 8.3, 20°C	Malassezia globosa
0.079	-	5-amino-1,3,4-thiadiazole-2-sulfonamide	pH 8.3, 20°C	Malassezia globosa
0.079	-	(4S,6S)-4-(ethylamino)-6-methyl-5,6-dihydro-4H-thieno[2,3-b]thiopyran-2-sulfonamide 7,7-dioxide	pH 8.3, 20°C	Malassezia globosa
0.084	-	4-sulfamoylbenzoic acid	pH 7.5, 20°C	Trypanosoma cruzi
0.084	-	(4R)-4-(ethylamino)-2-(3-methoxypropyl)-3,4-dihydro-2H-thieno[3,2-e][1,2]thiazine-6-sulfonamide 1,1-dioxide	pH 8.3, 20°C	Malassezia globosa
0.085	-	5-imino-4-methyl-4,5-dihydro-1,3,4-thiadiazole-2-sulfonamide	pH 8.3, 20°C	Malassezia globosa

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
69000	-	CO2	pH 8.3, 20°C	Malassezia globosa
149000	-	CO2	pH 7.5, 20°C	Trypanosoma cruzi