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Literature summary for 4.2.1.1 extracted from

  • Boone, C.D.; Habibzadegan, A.; Tu, C.; Silverman, D.N.; McKenna, R.
    Structural and catalytic characterization of a thermally stable and acid-stable variant of human carbonic anhydrase II containing an engineered disulfide bond (2013), Acta Crystallogr. Sect. D, 69, 1414-1422.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
X-ray crystallographic structure of the disulfide-containing HCAII (dsHCAII) is solved to 1.77 A resolution Homo sapiens

Protein Variants

Protein Variants Comment Organism
A23C/L203C/C206S mutant retains high catalytic efficiency, and differential scanning calorimetry shows acid stability and thermal stability that is enhanced compared with native enzyme Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P00918
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CO2 + H2O
-
 Homo sapiens H2CO3
-
 ?

Synonyms

Synonyms Comment Organism
hCAII
-
 Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45.5
-
 Tm, wild-type, pH 5.6 Homo sapiens
57.7
-
 Tm, wild-type, pH 7.8 Homo sapiens
59.7
-
 Tm, mutant A23C/L203C/C206S, pH 5.6 Homo sapiens
71.1
-
 Tm, mutant A23C/L203C/C206S, pH 7.8 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
120000
-
 CO2 pH 7.6, temperature not specified in the publication, wild-type Homo sapiens
130000
-
 CO2 pH 7.6, temperature not specified in the publication, mutant A23C/L203C/C206S Homo sapiens