Activating Compound | Comment | Organism | Structure |
---|---|---|---|
air | the enzyme loses activity when it was exposed to air | Methanosarcina thermophila | |
H2O2 | the enzyme loses activity when it was exposed to 3% H2O2 | Methanosarcina thermophila | |
imidazole | addition of 50 mM imidazole to the assay buffer increases the kcat of the enzyme more than 4fold | Methanosarcina thermophila |
Cloned (Comment) | Organism |
---|---|
overproduced in Escherichia coli | Methanosarcina thermophila |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | - |
CO2 | pH 7.5, 25°C, enzyme cultured with supplemental iron | Methanosarcina thermophila | |
3.1 | - |
CO2 | pH 7.5, 25°C, enzyme cultured with supplemental zinc | Methanosarcina thermophila |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the homotrimer purified from Escherichia coli cultured with supplemental zinc contains 0.71 zinc and 0.15 iron per monomer. Enzyme purified from Escheria coli cultured with supplemental iron contains 0.15 iron per monomer and only a trace amount of zinc | Methanosarcina thermophila | |
Zn2+ | the homotrimer purified from Escherichia coli cultured with supplemental zinc contains 0.71 zinc and 0.15 iron per monomer. Enzyme purified from Escherichia coli cultured with supplemental iron contains 0.15 iron per monomer and only a trace amount of zinc | Methanosarcina thermophila |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
2 * 20000, SDS-PAGE | Methanosarcina thermophila |
62000 | - |
gel filtration | Methanosarcina thermophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina thermophila | C3W4Q7 | - |
- |
Methanosarcina thermophila TM-1 | C3W4Q7 | - |
- |
Oxidation Stability | Organism |
---|---|
the enzyme loses activity when it is exposed to air or 3% H2O2 | Methanosarcina thermophila |
Purification (Comment) | Organism |
---|---|
- |
Methanosarcina thermophila |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:acetate-grown cell | the expression of the gene encoding CamH is substantially greater in acetate-grown cells than in methanol- or trimethylamine-grown cells | Methanosarcina thermophila | - |
culture condition:methanol-grown cell | the expression of the gene encoding CamH is substantially greater in acetate-grown cells than in methanol- or trimethylamine-grown cells | Methanosarcina thermophila | - |
culture condition:trimethylamine-grown cell | the expression of the gene encoding CamH is substantially greater in acetate-grown cells than in methanol- or trimethylamine-grown cells | Methanosarcina thermophila | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO2 + H2O | - |
Methanosarcina thermophila | H2CO3 | - |
? | |
CO2 + H2O | - |
Methanosarcina thermophila TM-1 | H2CO3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotrimer | 2 * 20000, SDS-PAGE | Methanosarcina thermophila |
Synonyms | Comment | Organism |
---|---|---|
CamH | - |
Methanosarcina thermophila |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Methanosarcina thermophila |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000493 | - |
CO2 | pH 7.5, 25°C, enzyme cultured with supplemental zinc | Methanosarcina thermophila | |
0.0052 | - |
CO2 | pH 7.5, 25°C, enzyme cultured with supplemental iron | Methanosarcina thermophila |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Methanosarcina thermophila |
Organism | Comment | Expression |
---|---|---|
Methanosarcina thermophila | the expression of the gene encoding CamH is substantially greater in acetate-grown cells than in methanol- or trimethylamine-grown cells | up |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00016 | - |
CO2 | pH 7.5, 25°C, enzyme cultured with supplemental zinc | Methanosarcina thermophila | |
0.0017 | - |
CO2 | pH 7.5, 25°C, enzyme cultured with supplemental iron | Methanosarcina thermophila |