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Literature summary for 4.2.1.1 extracted from

  • Zimmerman, S.A.; Tomb, J.F.; Ferry, J.G.
    Characterization of CamH from Methanosarcina thermophila, founding member of a subclass of the gamma class of carbonic anhydrases (2010), J. Bacteriol., 192, 1353-1360.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
air the enzyme loses activity when it was exposed to air Methanosarcina thermophila
H2O2 the enzyme loses activity when it was exposed to 3% H2O2 Methanosarcina thermophila
imidazole addition of 50 mM imidazole to the assay buffer increases the kcat of the enzyme more than 4fold Methanosarcina thermophila

Cloned(Commentary)

Cloned (Comment) Organism
overproduced in Escherichia coli Methanosarcina thermophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3
-
CO2 pH 7.5, 25°C, enzyme cultured with supplemental iron Methanosarcina thermophila
3.1
-
CO2 pH 7.5, 25°C, enzyme cultured with supplemental zinc Methanosarcina thermophila

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ the homotrimer purified from Escherichia coli cultured with supplemental zinc contains 0.71 zinc and 0.15 iron per monomer. Enzyme purified from Escheria coli cultured with supplemental iron contains 0.15 iron per monomer and only a trace amount of zinc Methanosarcina thermophila
Zn2+ the homotrimer purified from Escherichia coli cultured with supplemental zinc contains 0.71 zinc and 0.15 iron per monomer. Enzyme purified from Escherichia coli cultured with supplemental iron contains 0.15 iron per monomer and only a trace amount of zinc Methanosarcina thermophila

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
2 * 20000, SDS-PAGE Methanosarcina thermophila
62000
-
gel filtration Methanosarcina thermophila

Organism

Organism UniProt Comment Textmining
Methanosarcina thermophila C3W4Q7
-
-
Methanosarcina thermophila TM-1 C3W4Q7
-
-

Oxidation Stability

Oxidation Stability Organism
the enzyme loses activity when it is exposed to air or 3% H2O2 Methanosarcina thermophila

Purification (Commentary)

Purification (Comment) Organism
-
Methanosarcina thermophila

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:acetate-grown cell the expression of the gene encoding CamH is substantially greater in acetate-grown cells than in methanol- or trimethylamine-grown cells Methanosarcina thermophila
-
culture condition:methanol-grown cell the expression of the gene encoding CamH is substantially greater in acetate-grown cells than in methanol- or trimethylamine-grown cells Methanosarcina thermophila
-
culture condition:trimethylamine-grown cell the expression of the gene encoding CamH is substantially greater in acetate-grown cells than in methanol- or trimethylamine-grown cells Methanosarcina thermophila
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CO2 + H2O
-
Methanosarcina thermophila H2CO3
-
?
CO2 + H2O
-
Methanosarcina thermophila TM-1 H2CO3
-
?

Subunits

Subunits Comment Organism
heterotrimer 2 * 20000, SDS-PAGE Methanosarcina thermophila

Synonyms

Synonyms Comment Organism
CamH
-
Methanosarcina thermophila

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Methanosarcina thermophila

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000493
-
CO2 pH 7.5, 25°C, enzyme cultured with supplemental zinc Methanosarcina thermophila
0.0052
-
CO2 pH 7.5, 25°C, enzyme cultured with supplemental iron Methanosarcina thermophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Methanosarcina thermophila

Expression

Organism Comment Expression
Methanosarcina thermophila the expression of the gene encoding CamH is substantially greater in acetate-grown cells than in methanol- or trimethylamine-grown cells up

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00016
-
CO2 pH 7.5, 25°C, enzyme cultured with supplemental zinc Methanosarcina thermophila
0.0017
-
CO2 pH 7.5, 25°C, enzyme cultured with supplemental iron Methanosarcina thermophila