Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.1.1 extracted from

  • Joseph, P.; Turtaut, F.; Ouahrani-Bettache, S.; Montero, J.L.; Nishimori, I.; Minakuchi, T.; Vullo, D.; Scozzafava, A.; Koehler, S.; Winum, J.Y.; Supuran, C.T.
    Cloning, characterization, and inhibition studies of a beta-carbonic anhydrase from Brucella suis (2010), J. Med. Chem., 53, 2277-2285.
    View publication on PubMed
Search for more literature for 4.2.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Brucella suis

Inhibitors

Inhibitors Comment Organism Structure
2-aminobenzenesulfonamide
-
 Brucella suis
2-hydrazinylbenzenesulfonamide
-
 Brucella suis
4,5-dichloro-benzene-1,3-disulfonamide
-
 Brucella suis
4-(2-aminoethyl)benzenesulfonamide
-
 Brucella suis
4-(2-hydroxyethyl)benzenesulfonamide
-
 Brucella suis
4-(aminomethyl)benzenesulfonamide
-
 Brucella suis
4-(hydroxymethyl)benzenesulfonamide
-
 Brucella suis
4-amino-3,5-dibromobenzenesulfonamide
-
 Brucella suis
4-amino-3,5-diiodobenzenesulfonamide
-
 Brucella suis
4-amino-3-chlorobenzenesulfonamide
-
 Brucella suis
4-amino-3-fluorobenzenesulfonamide
-
 Brucella suis
4-amino-6-(trifluoromethyl)benzene-1,3-disulfonamide
-
 Brucella suis
4-amino-6-chlorobenzene-1,3-disulfonamide
-
 Brucella suis
4-amino-N-(4-sulfamoylbenzyl)benzenesulfonamide
-
 Brucella suis
4-amino-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide
-
 Brucella suis
4-Aminobenzenesulfonamide
-
 Brucella suis
4-hydrazinylbenzenesulfonamide
-
 Brucella suis
4-methylbenzenesulfonamide
-
 Brucella suis
4-sulfamoyl-N-[2-(4-sulfamoylphenyl)ethyl]benzamide
-
 Brucella suis
4-sulfamoylbenzoic acid
-
 Brucella suis
4-[(2-aminopyrimidin-4-yl)amino]benzenesulfonamide
-
 Brucella suis
5-(2-chlorophenyl)-1,3,4-thiadiazole-2-sulfonamide
-
 Brucella suis
5-amino-1,3,4-thiadiazole-2-sulfonamide
-
 Brucella suis
5-imino-4-methyl-4,5-dihydro-1,3,4-thiadiazole-2-sulfonamide
-
 Brucella suis
5-[[(4-amino-3-chloro-5-fluorophenyl)sulfonyl]amino]-1,3,4-thiadiazole-2-sulfonamide
-
 Brucella suis
5-[[(4-aminophenyl)sulfonyl]amino]-1,3,4-thiadiazole-2-sulfonamide
-
 Brucella suis
6-hydroxy-1,3-benzothiazole-2-sulfonamide
-
 Brucella suis
acetazolamide
-
 Brucella suis
benzolamide
-
 Brucella suis
brinzolamide
-
 Brucella suis
celecoxib best CA 1 inhibitor Brucella suis
dorzolamide best CA 1 inhibitor Brucella suis
ethoxzolamide best CA 1 inhibitor Brucella suis
indisulam
-
 Brucella suis
methazolamide
-
 Brucella suis
sulpiride best CA 1 inhibitor Brucella suis
topiramate
-
 Brucella suis
valdecoxib best CA 1 inhibitor Brucella suis
zonisamide
-
 Brucella suis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 2 * 25000, SDS-PAGE Brucella suis

Organism

Organism UniProt Comment Textmining
Brucella suis A0A0H3GB68
-
-

Purification (Commentary)

Purification (Comment) Organism
Talon Co2+-affinity resin column chromatography, gel filtration Brucella suis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CO2 + H2O
-
 Brucella suis H2CO3
-
 r

Subunits

Subunits Comment Organism
homodimer 2 * 25000, SDS-PAGE Brucella suis

Synonyms

Synonyms Comment Organism
beta-carbonic anhydrase
-
 Brucella suis
CA 1
-
 Brucella suis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
640000
-
 CO2 in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000017
-
 ethoxzolamide in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
0.000018
-
 celecoxib in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
0.000019
-
 valdecoxib in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
0.000019
-
 sulpiride in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
0.000021
-
 dorzolamide in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
0.000021
-
 4-[(2-aminopyrimidin-4-yl)amino]benzenesulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
0.000027
-
 4-sulfamoyl-N-[2-(4-sulfamoylphenyl)ethyl]benzamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
0.000033
-
 5-(2-chlorophenyl)-1,3,4-thiadiazole-2-sulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
0.000048
-
 6-hydroxy-1,3-benzothiazole-2-sulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
0.000063
-
 acetazolamide in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
0.00007
-
 5-[[(4-aminophenyl)sulfonyl]amino]-1,3,4-thiadiazole-2-sulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
0.000186
-
 5-[[(4-amino-3-chloro-5-fluorophenyl)sulfonyl]amino]-1,3,4-thiadiazole-2-sulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
0.000243
-
 4-amino-3,5-dibromobenzenesulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
0.00034
-
 4-sulfamoylbenzoic acid isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
0.000345
-
 4-amino-3,5-diiodobenzenesulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
0.000745
-
 4-amino-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
0.000754
-
 4-(hydroxymethyl)benzenesulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
0.000768
-
 4-(aminomethyl)benzenesulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
0.0008
-
 4-amino-3-chlorobenzenesulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
0.000865
-
 4-(2-hydroxyethyl)benzenesulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
0.00088
-
 4-(2-aminoethyl)benzenesulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
0.00094
-
 4-amino-6-chlorobenzene-1,3-disulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
0.001035
-
 2-hydrazinylbenzenesulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
0.00105
-
 4-amino-N-(4-sulfamoylbenzyl)benzenesulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
0.00107
-
 4-amino-3-fluorobenzenesulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
0.00121
-
 5-amino-1,3,4-thiadiazole-2-sulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
0.00143
-
 5-imino-4-methyl-4,5-dihydro-1,3,4-thiadiazole-2-sulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
0.00158
-
 4-methylbenzenesulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
0.0024
-
 4-hydrazinylbenzenesulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
0.0025
-
 4-Aminobenzenesulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
0.00483
-
 4-amino-6-(trifluoromethyl)benzene-1,3-disulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis
0.00587
-
 2-aminobenzenesulfonamide isoform CA I, in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
39000
-
 CO2 in 10-20 mM Tris, pH 8.3, 10-20 mM NaCl Brucella suis