Literature summary for 4.2.1.1 extracted from

De Simone, G.; Supuran, C.T.
Carbonic anhydrase IX: Biochemical and crystallographic characterization of a novel antitumor target (2010), Biochim. Biophys. Acta, 1804, 404-409.

Search for more literature for 4.2.1.1

Application

Application	Comment	Organism
medicine	isoform CA IX is used as a marker of tumor hypoxia and as a prognostic factor for many human cancers	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells and in insect cells	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
5-acetamido-1,3,4-thiadiazole-2-sulfonamide	-	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
6.9	-	CO2	Escherichia coli recombinant enzyme	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	transmembrane protein	Homo sapiens	16020	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	zinc-containing enzyme	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
CO2 + H2O	-	Homo sapiens	H2CO3	-	r

Subunits

Subunits	Comment	Organism
dimer	two catalytic domains of CA IX associate to form a dimer, which is stabilized by the formation of an intermolecular disulfide bond	Homo sapiens

Synonyms

Synonyms	Comment	Organism
CA IX	-	Homo sapiens
carbonic anhydrase IX	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
380000	-	CO2	Escherichia coli recombinant enzyme	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.025	-	5-acetamido-1,3,4-thiadiazole-2-sulfonamide	Escherichia coli recombinant enzyme	Homo sapiens

Expression

Organism	Comment	Expression
Homo sapiens	expression of CA IX is strongly increased in many types of tumors, such as gliomas/ependymomas, mesotheliomas, and papillary/follicular carcinomas, as well as carcinomas of the bladder, uterine cervix, kidneys, esophagus, lungs, head and neck, breast, brain, vulva, and squamous/basal cell carcinomas	up

General Information

General Information	Comment	Organism
physiological function	isoform CA IX is involved in tumorigenesis through many pathways, such as pH regulation and cell adhesion control	Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
55000	-	CO2	Escherichia coli recombinant enzyme	Homo sapiens

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Release 2023.1 (January 2023)