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Literature summary for 4.2.1.1 extracted from
- Purification and properties of carbonic anhydrase from bone marrow (2009), Asian J. Chem., 21, 5117-5122.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetazolamide | uncompetitive inhibitor | Bos taurus |  |
| KSCN | uncompetitive inhibitor | Bos taurus | |
| NaN3 | uncompetitive inhibitor | Bos taurus | |
| sulfanilamide | uncompetitive inhibitor | Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002409 | - |
4-nitrophenyl acetate | at pH 7.0 and 20°C | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
gel filtration | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CO2 + H2O | Bos taurus | - |
H2CO3 | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Sepharose 4B-L-tyrosine sulfanilamide column chromatography | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| bone marrow | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.052 | - |
crude homogenate, at 20°C | Bos taurus |
| 7.843 | - |
after 150.82fold purification, at 20°C | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl acetate + H2O | - |
Bos taurus | 4-nitrophenol + acetate | - |
? | |
| CO2 + H2O | - |
Bos taurus | H2CO3 | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| carbonic anhydrase | - |
Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Bos taurus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 70 | - |
Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 8 | - |
Bos taurus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00000718 | - |
KSCN | at pH 7.0 and 20°C | Bos taurus | |
| 0.0000212 | - |
acetazolamide | at pH 7.0 and 20°C | Bos taurus | |
| 0.0000343 | - |
NaN3 | at pH 7.0 and 20°C | Bos taurus | |
| 0.0000521 | - |
sulfanilamide | at pH 7.0 and 20°C | Bos taurus | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.824 | - |
at pH 7.0 and 20°C | Bos taurus | sulfanilamide | |
| 1.145 | - |
at pH 7.0 and 20°C | Bos taurus | acetazolamide | |
| 1.151 | - |
at pH 7.0 and 20°C | Bos taurus | KSCN | |
| 1.529 | - |
at pH 7.0 and 20°C | Bos taurus | NaN3 | |
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Release 2023.1 (January 2023)
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