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Literature summary for 4.2.1.1 extracted from
- Purification and some kinetic properties of carbonic anhydrase from rainbow trout (Oncorhynchus mykiss) liver and metal inhibition (2008), Protein Pept. Lett., 15, 528-535.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | uncompetitive inhibition | Oncorhynchus mykiss |  |
| Co2+ | competitive inhibition | Oncorhynchus mykiss | |
| Cu2+ | uncompetitive inhibition | Oncorhynchus mykiss | |
| Zn2+ | competitive inhibition | Oncorhynchus mykiss | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29400 | - |
SDS-PAGE | Oncorhynchus mykiss |
| 31000 | - |
native enzyme, gel filtration | Oncorhynchus mykiss |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oncorhynchus mykiss | - |
rainbow trout | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Sepharose-4B-L-tyrosine-sulfanilamide affinity column chromatography | Oncorhynchus mykiss |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Oncorhynchus mykiss | - |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| carbonic anhydrase | - |
Oncorhynchus mykiss |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Oncorhynchus mykiss |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
in 1 M Tris-SO4 buffer at 4°C | Oncorhynchus mykiss |
| 9 | - |
in 1 M Tris-SO4 buffer | Oncorhynchus mykiss |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9 | in 1 M Tris-SO4 buffer | Oncorhynchus mykiss |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
Co2+ | - |
Oncorhynchus mykiss | |
| 1.95 | - |
Cu2+ | - |
Oncorhynchus mykiss | |
| 2.19 | - |
Ag+ | - |
Oncorhynchus mykiss | |
| 7.035 | - |
Zn2+ | - |
Oncorhynchus mykiss | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
- |
Oncorhynchus mykiss | Ag+ | |
| 0.03 | - |
- |
Oncorhynchus mykiss | Co2+ | |
| 30 | - |
- |
Oncorhynchus mykiss | Cu2+ | |
| 47.1 | - |
- |
Oncorhynchus mykiss | Zn2+ | |
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