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Literature summary for 4.2.1.1 extracted from

  • Engstrand, C.; Jonsson, B.H.; Lindskog, S.
    Catalytic and inhibitor-binding properties of some active-site mutants of human carbonic anhydrase I (1995), Eur. J. Biochem., 229, 696-702.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
H200T complex effect on the catalytic and inhibitor-binding properties Homo sapiens
H67N complex effect on the catalytic and inhibitor-binding properties Homo sapiens
V62H/H67N/H200T complex effect on the catalytic and inhibitor-binding properties Homo sapiens
V62N complex effect on the catalytic and inhibitor-binding properties Homo sapiens
V62N/H67N complex effect on the catalytic and inhibitor-binding properties Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
4-S-isobutylamino-5,6-dihydro-4H-thieno[2,3-b]thiopyran-2-sulfonamide 7,7-dioxide binds 800 times more strongly to carbonic anhydrase II than to carbonic anhydrase I Homo sapiens
I- inhibits 4-nitrophenyl acetate hydrolase activity Homo sapiens
SCN- inhibits 4-nitrophenyl acetate hydrolase activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.31
-
CO2 pH 8.95, 1,2-dimethylimidazole buffer, carbonic anhydrase I mutant H200T Homo sapiens
2.3
-
CO2 pH 8.98, TAPS buffer, carbonic anhydrase I mutant V62N,H67N, H200T Homo sapiens
2.4
-
CO2 pH 7.21, 1-methylimidazole buffer, carbonic anhydrase I mutant H200T Homo sapiens
2.4
-
CO2 pH 8.98, TAPS buffer, carbonic anhydrase I mutant H67B Homo sapiens
2.8
-
CO2 pH 8.94, 1,2-dimethylimidazole buffer, carbonic anhydrase II mutant N62V Homo sapiens
3.5
-
CO2 pH 8.98, TAPS buffer, carbonic anhydrase I mutant V62N Homo sapiens
3.6
-
CO2 pH 6.28, MES buffer, carbonic anhydrase I mutant V62N,H67N,H200T Homo sapiens
3.6
-
CO2 pH 7.21, 1-methylimidazole buffer, wild-type carbonic anhydrase I Homo sapiens
3.9
-
CO2 pH 8.94, 1,2-dimethylimidazole buffer, carbonic anhydrase II mutant T200H Homo sapiens
4
-
CO2 pH 8.88, TAPS buffer, carbonic anhydrase I mutant V62N,H67N Homo sapiens
5.3
-
CO2 pH 8.77, 1,2-dimethylimidazole buffer, wild-type carbonic anhydrase I Homo sapiens
6.6
-
CO2 pH 8.75, 1,2-dimethylimidazole buffer, carbonic anhydrase I mutant H67N Homo sapiens
7.1
-
CO2 pH 8.95, 1,2-dimethylimidazole buffer, carbonic anhydrase I mutant V62N Homo sapiens
7.5
-
CO2 pH 7.23, 1-methylimidazolebuffer, carbonic anhydrase I mutant V62N,H67N,H200T Homo sapiens
8.8
-
CO2 pH 8.7, 1,2-dimethylimidazole buffer, carbonic anhydrase II Homo sapiens
9.4
-
CO2 pH 8.72, 1,2-dimethyimidazole buffer, carbonic anhydrase I mutant V62N,H67N Homo sapiens
9.7
-
CO2 pH 8.9, TAPS buffer, carbonic anhydrase II Homo sapiens
10
-
CO2 pH 8.95, 1,2-dimethylimidazole buffer, carbonic anhydrase I mutant H200T Homo sapiens
14
-
CO2 pH 8.75, 1,2-dimethylimidazole buffer, carbonic anhydrase I mutant V62N,H67N,H200T Homo sapiens
14
-
CO2 pH 8.94, 1,2-dimethylimidazole buffer, carbonic anhydrase II mutant N67HV Homo sapiens
15
-
CO2 pH 7.23, 1-methylimidazole buffer, carbonic anhydrase I mutant H67N Homo sapiens
46
-
CO2 pH 6.28, MES buffer, carbonic anhydrase I mutant H67N Homo sapiens
69
-
CO2 pH 6.28, MES buffer, carbonic anhydrase I mutant V62N Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
wild-type carbonic anhydrase I and active-site mutants V62N, H67N, H200T, double-mutant V62N,H67N, and triple-mutant V62H,H67N,H200T. Wild-type carbonic anhydrase II and active site mutant enzymes N62V, N67H and T200H
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CO2 + H2O
-
Homo sapiens H2CO3
-
?
additional information esterase activity with p-nitrophenyl acetate Homo sapiens ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
85
-
CO2 pH 6.28, MES buffer, carbonic anhydrase I mutant V62N,H67N,H200T Homo sapiens
117
-
CO2 pH 6.3, MES buffer, wild-type carbonic anhydrase I Homo sapiens
217
-
CO2 pH 6.28, MES buffer, carbonic anhydrase I mutant V62N Homo sapiens
217
-
CO2 pH 6.3, MES buffer Homo sapiens
233
-
CO2 pH 8.88, TAPS buffer, carbonic anhydrase I mutant H200T Homo sapiens
250
-
CO2 pH 6.28, MES buffer, carbonic anhydrase I mutant H67N Homo sapiens
433
-
CO2 pH 8.8, TAPS buffer, wild-type carbonic anhydrase I Homo sapiens
800
-
CO2 pH 7.21, 1-methylimidazole buffer, wild-type carbonic anhydrase I Homo sapiens
883
-
CO2 pH 7.23, 1-methylimidazole buffer, carbonic anhydrase I mutant H67N Homo sapiens
1180
-
CO2 pH 8.98, TAPS buffer, carbonic anhydrase I mutant V62N and carbonic anhydrase I mutant H67N Homo sapiens
1380
-
CO2 pH 7.21, 1-methylimidazole buffer, carbonic anhydrase I mutant H200T Homo sapiens
1400
-
CO2 pH 8.98, TAPS buffer, carbonic anhydrase I mutant V62N,H67N,H200T Homo sapiens
1470
-
CO2 pH 8.88, TAPS buffer, carbonic anhydrase I mutant V62N,H67N Homo sapiens
2000
-
CO2 pH 8.95, 1,2-dimethylimidazole buffer, carbonic anhydrase I mutant V62N Homo sapiens
2830
-
CO2 pH 8.75, 1,2-dimethylimidazole buffer, carbonic anhydrase I mutant H67N Homo sapiens
3500
-
CO2 pH 8.77, 1,2-dimethylimidazole buffer, wild-type carbonic anhydrase I Homo sapiens
3500
-
CO2 pH 8.72, 1,2-dimethylimidazole buffer, carbonic anhydrase I mutant V62B, H67B Homo sapiens
3670
-
CO2 pH 7.23, 1-methylimidazolebuffer, carbonic anhydrase I mutant V62N,H67N,H200T Homo sapiens
4000
-
CO2 pH 8.94, 1,2-dimethylimidazole buffer, carbonic anhydrase II mutant T200H Homo sapiens
5500
-
CO2 pH 8.95, 1,2-dimethylimidazole buffer, carbonic anhydrase I mutant H200T Homo sapiens
8170
-
CO2 pH 8.94, 1,2-dimethylimidazole buffer, carbonic anhydrase II mutant N62V Homo sapiens
8500
-
CO2 pH 8.75, 1,2-dimethylimidazole buffer, carbonic anhydrase I mutant V62N,H67N,H200T Homo sapiens
15200
-
CO2 pH 8.7, 1,2-dimethylimidazole buffer, carbonic anhydrase II Homo sapiens
16700
-
CO2 pH 8.9, TAPS buffer, carbonic anhydrase II Homo sapiens
28300
-
CO2 pH 8.94, 1,2-dimethylimidazole buffer, carbonic anhydrase II mutant N67H Homo sapiens