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Literature summary for 4.1.99.5 extracted from
- Identification of residues important for the activity of aldehyde-deformylating oxygenase through investigation into the structure-activity relationship (2017), BMC Biotechnol., 17, 31-39 .
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D143A | site-directed mutagenesis, altered activity compared to wild-type | Synechococcus elongatus PCC 7942 = FACHB-805 |
| D49H | site-directed mutagenesis, altered activity compared to wild-type | Synechocystis sp. PCC 6803 |
| D49H | site-directed mutagenesis, altered activity compared to wild-type | Synechococcus elongatus PCC 7942 = FACHB-805 |
| D49H/N123H | site-directed mutagenesis, altered activity compared to wild-type | Synechocystis sp. PCC 6803 |
| F150Y | site-directed mutagenesis, altered activity compared to wild-type | Synechococcus elongatus PCC 7942 = FACHB-805 |
| L146T | site-directed mutagenesis, altered activity compared to wild-type | Synechococcus elongatus PCC 7942 = FACHB-805 |
| L148R | site-directed mutagenesis, altered activity compared to wild-type | Synechocystis sp. PCC 6803 |
| additional information | cADO is engineered to improve specificity for short- to medium-chain aldehydes, site-directed mutagenesis of some residues in analogy to the more active enzyme from Prochlorococcus marinus strain MIT9313 | Synechocystis sp. PCC 6803 |
| additional information | cADO is engineered to improve specificity for short- to medium-chain aldehydes, site-directed mutagenesis of some residues in analogy to the more active enzyme from Prochlorococcus marinus strain MIT9313 | Synechococcus elongatus PCC 7942 = FACHB-805 |
| N123H | site-directed mutagenesis, altered activity compared to wild-type | Synechocystis sp. PCC 6803 |
| N123H | site-directed mutagenesis, altered activity compared to wild-type | Synechococcus elongatus PCC 7942 = FACHB-805 |
| N149A | site-directed mutagenesis, altered activity compared to wild-type | Synechococcus elongatus PCC 7942 = FACHB-805 |
| Q110L | site-directed mutagenesis, altered activity compared to wild-type | Synechococcus elongatus PCC 7942 = FACHB-805 |
| Q49H/F150Y | site-directed mutagenesis, altered activity compared to wild-type | Synechococcus elongatus PCC 7942 = FACHB-805 |
| Q49H/N123H/F150Y | site-directed mutagenesis, altered activity compared to wild-type | Synechococcus elongatus PCC 7942 = FACHB-805 |
| R62A | site-directed mutagenesis, altered activity compared to wild-type | Synechococcus elongatus PCC 7942 = FACHB-805 |
| T146L | site-directed mutagenesis, altered activity compared to wild-type | Synechocystis sp. PCC 6803 |
| W178R | site-directed mutagenesis, altered activity compared to wild-type | Synechococcus elongatus PCC 7942 = FACHB-805 |
| Y122F | site-directed mutagenesis, altered activity compared to wild-type | Synechococcus elongatus PCC 7942 = FACHB-805 |
| Y150F | site-directed mutagenesis, altered activity compared to wild-type | Synechocystis sp. PCC 6803 |
| Y39F | site-directed mutagenesis, altered activity compared to wild-type | Synechococcus elongatus PCC 7942 = FACHB-805 |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
n-Heptanal | recombinant mutant F150Y, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 |  |
| 0.3 | - |
n-Heptanal | recombinant wild-type enzyme, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| 0.32 | - |
n-Heptanal | recombinant mutant L148R, pH and temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
| 0.33 | - |
n-Heptanal | recombinant mutant Q49H/F150Y, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| 0.34 | - |
n-Heptanal | recombinant mutant L146T, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| 0.34 | - |
n-Heptanal | recombinant mutant W178R, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| 0.35 | - |
n-Heptanal | recombinant wild-type enzyme, pH and temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
| 0.35 | - |
n-Heptanal | recombinant mutant Q49H/N123H/F150Y, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| 0.38 | - |
n-Heptanal | recombinant mutant D49H/N123H, pH and temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | di-iron center | Synechocystis sp. PCC 6803 | |
| Fe2+ | di-iron center | Synechococcus elongatus PCC 7942 = FACHB-805 | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| a long-chain aldehyde + O2 + 2 NADPH + 2 H+ | Synechocystis sp. PCC 6803 | - |
an alkane + formate + H2O + 2 NADP+ | - |
? | |
| a long-chain aldehyde + O2 + 2 NADPH + 2 H+ | Synechococcus elongatus PCC 7942 = FACHB-805 | - |
an alkane + formate + H2O + 2 NADP+ | - |
? | |
| a long-chain aldehyde + O2 + 2 NADPH + 2 H+ | Synechococcus elongatus PCC 7942 = FACHB-805 R2 | - |
an alkane + formate + H2O + 2 NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Synechococcus elongatus PCC 7942 = FACHB-805 | Q54764 | i.e. Synechococcus elongates strain PCC 7942 | - |
| Synechococcus elongatus PCC 7942 = FACHB-805 R2 | Q54764 | i.e. Synechococcus elongates strain PCC 7942 | - |
| Synechocystis sp. PCC 6803 | Q55688 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| a long-chain aldehyde + O2 + 2 NADPH + 2 H+ | - |
Synechocystis sp. PCC 6803 | an alkane + formate + H2O + 2 NADP+ | - |
? | |
| a long-chain aldehyde + O2 + 2 NADPH + 2 H+ | - |
Synechococcus elongatus PCC 7942 = FACHB-805 | an alkane + formate + H2O + 2 NADP+ | - |
? | |
| a long-chain aldehyde + O2 + 2 NADPH + 2 H+ | - |
Synechococcus elongatus PCC 7942 = FACHB-805 R2 | an alkane + formate + H2O + 2 NADP+ | - |
? | |
| additional information | cADO shows extreme low activity with kcat value below 1/min | Synechococcus elongatus PCC 7942 = FACHB-805 | ? | - |
? | |
| additional information | cADO shows extreme low activity with kcat value below 1/min | Synechococcus elongatus PCC 7942 = FACHB-805 R2 | ? | - |
? | |
| n-heptanal + O2 + 2 NADPH + 2 H+ | - |
Synechocystis sp. PCC 6803 | n-hexane + formate + H2O + 2 NADP+ | - |
? | |
| n-heptanal + O2 + 2 NADPH + 2 H+ | - |
Synechococcus elongatus PCC 7942 = FACHB-805 | n-hexane + formate + H2O + 2 NADP+ | - |
? | |
| n-heptanal + O2 + 2 NADPH + 2 H+ | - |
Synechococcus elongatus PCC 7942 = FACHB-805 R2 | n-hexane + formate + H2O + 2 NADP+ | - |
? | |
| n-hexadecanal + O2 + 2 NADPH + 2 H+ | - |
Synechocystis sp. PCC 6803 | pentadecane + formate + H2O + 2 NADP+ | - |
? | |
| n-hexadecanal + O2 + 2 NADPH + 2 H+ | - |
Synechococcus elongatus PCC 7942 = FACHB-805 | pentadecane + formate + H2O + 2 NADP+ | - |
? | |
| n-hexadecanal + O2 + 2 NADPH + 2 H+ | - |
Synechococcus elongatus PCC 7942 = FACHB-805 R2 | pentadecane + formate + H2O + 2 NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | enzyme structure comparisons, overview | Synechococcus elongatus PCC 7942 = FACHB-805 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| aldehyde decarbonylase | UniProt | Synechococcus elongatus PCC 7942 = FACHB-805 |
| aldehyde-deformylating oxygenase | - |
Synechocystis sp. PCC 6803 |
| aldehyde-deformylating oxygenase | - |
Synechococcus elongatus PCC 7942 = FACHB-805 |
| cADO | - |
Synechocystis sp. PCC 6803 |
| cADO | - |
Synechococcus elongatus PCC 7942 = FACHB-805 |
| sll0208 | - |
Synechocystis sp. PCC 6803 |
| Synpcc7942_1593 | locus name | Synechococcus elongatus PCC 7942 = FACHB-805 |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0007 | - |
n-hexadecanal | recombinant mutant R62A, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| 0.0023 | - |
n-hexadecanal | recombinant mutant N123H, pH and temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
| 0.0025 | - |
n-hexadecanal | recombinant mutant Y122F, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| 0.0028 | - |
n-hexadecanal | recombinant mutant T146L, pH and temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
| 0.0037 | - |
n-hexadecanal | recombinant mutant D49H, pH and temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
| 0.0038 | - |
n-hexadecanal | recombinant mutant Q110L, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| 0.0052 | - |
n-hexadecanal | recombinant mutant N149A, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| 0.006 | - |
n-hexadecanal | recombinant mutant D143A, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| 0.0062 | - |
n-hexadecanal | recombinant mutant Y39F, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| 0.007 | - |
n-hexadecanal | recombinant mutant Y150F, pH and temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
| 0.0072 | - |
n-hexadecanal | recombinant mutant N123H, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| 0.0073 | - |
n-hexadecanal | recombinant wild-type enzyme, pH and temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
| 0.0075 | - |
n-hexadecanal | recombinant wild-type enzyme, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| 0.008 | - |
n-Heptanal | recombinant wild-type enzyme, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| 0.0098 | - |
n-Heptanal | recombinant wild-type enzyme, pH and temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
| 0.012 | - |
n-hexadecanal | recombinant mutant Q49H/F150Y, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| 0.0122 | - |
n-hexadecanal | recombinant mutant D49H/N123H, pH and temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
| 0.0125 | - |
n-Heptanal | recombinant mutant F150Y, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| 0.0125 | - |
n-hexadecanal | recombinant mutant L148R, pH and temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
| 0.0127 | - |
n-hexadecanal | recombinant mutant F150Y, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| 0.0138 | - |
n-Heptanal | recombinant mutant D49H/N123H, pH and temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
| 0.0145 | - |
n-hexadecanal | recombinant mutant L146T, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| 0.0145 | - |
n-Heptanal | recombinant mutant Q49H/F150Y, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| 0.0145 | - |
n-hexadecanal | recombinant mutant Q49H/N123H/F150Y, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| 0.0152 | - |
n-Heptanal | recombinant mutant L148R, pH and temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
| 0.0157 | - |
n-Heptanal | recombinant mutant L146T, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| 0.0173 | - |
n-Heptanal | recombinant mutant Q49H/N123H/F150Y, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| 0.0245 | - |
n-hexadecanal | recombinant mutant W178R, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| 0.0302 | - |
n-Heptanal | recombinant mutant W178R, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Synechococcus elongatus PCC 7942 = FACHB-805 | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | comparison of the enzyme from Synechococcus elongates strain PCC 7942 and Synechocystis sp. PCC 6803, the first is more active than the latter against n-hexadecanal. Enzyme structure-function relationship analysis and comparisons, overview | Synechocystis sp. PCC 6803 |
| additional information | residues close to the di-iron center (Tyr39, Gln110, Tyr122), the protein surface (Trp178), and involved in the hydrogen-bonding network (Arg62, Asp143) and the oligopeptide whose conformation changed (Leu/Thr146, Leu148, Asn149 and Tyr/Phe150) in the absence of the diiron center are identified. Comparison of the enzyme from Synechococcus elongates strain PCC 7942 and Synechocystis sp. PCC 6803, the first is more active than the latter against n-hexadecanal. Enzyme structure-function relationship analysis and comparisons, overview | Synechococcus elongatus PCC 7942 = FACHB-805 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0267 | - |
n-Heptanal | recombinant wild-type enzyme, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| 0.0282 | - |
n-Heptanal | recombinant wild-type enzyme, pH and temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
| 0.0363 | - |
n-Heptanal | recombinant mutant D49H/N123H, pH and temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
| 0.044 | - |
n-Heptanal | recombinant mutant Q49H/F150Y, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| 0.046 | - |
n-Heptanal | recombinant mutant L146T, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| 0.0473 | - |
n-Heptanal | recombinant mutant L148R, pH and temperature not specified in the publication | Synechocystis sp. PCC 6803 | |
| 0.0495 | - |
n-Heptanal | recombinant mutant Q49H/N123H/F150Y, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| 0.0625 | - |
n-Heptanal | recombinant mutant F150Y, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| 0.0887 | - |
n-Heptanal | recombinant mutant W178R, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
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