Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the Nostoc punctiforme enzyme is triggered by short-chain (C6-C10) n-aldehyde substrates4 for addition of O2 to its Fe2 II/II cofactor | Nostoc punctiforme |
Cloned (Comment) | Organism |
---|---|
gene Npun_R1711, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Nostoc punctiforme |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | complex kinetics of formation of the reaction intermediate, overview | Nostoc punctiforme |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | di-iron enzyme, Moessbauer spectrum of the cofactor during the reaction steps, coordinations of the different cofactor states, e.g. the peroxo-Fe2 III/III intermediate, Fe2 III/III peroxyhemiacetal complex, or a micro-oxo-Fe2 III/III cluster, detailed overview | Nostoc punctiforme |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Nostoc punctiforme | cyanobacterial aldehyde-deformylating oxygenases catalyze conversion of saturated or monounsaturated Cn fatty aldehydes to formate and the corresponding Cn-1 alkanes or alkenes, respectively | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nostoc punctiforme | B2J1M1 | gene Npun_R1711 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) in an anaerobic chamber, further by metal affinity chromatography | Nostoc punctiforme |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
octadecanal + O2 + 2 NADPH + 2 H+ = heptadecane + formate + H2O + 2 NADP+ | the catalytic mechanism involves attack on the carbonyl of the bound substrate by the reduced O2 moiety to form a Fe2 III/III peroxyhemiacetal complex, which undergoes reductive O-O-bond cleavage, leading to C1-C2 radical fragmentation and formation of the alk(a/e)ne and formate products, detailed overview | Nostoc punctiforme |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | cyanobacterial aldehyde-deformylating oxygenases catalyze conversion of saturated or monounsaturated Cn fatty aldehydes to formate and the corresponding Cn-1 alkanes or alkenes, respectively | Nostoc punctiforme | ? | - |
? | |
octanal + O2 + 2 NADH + 2 H+ | with reducing system NADH/phenazine methosulfate, reaction under anaerobic conditions to protect the cofactor, but the enzyme shows no differences between aerobic and anaerobic condition, meaning that the substrate does not bind tightly to the Fe2 III/III form of the enzyme or that the aldehyde binds in a manner that does not detectably alter its Moessbauer properties | Nostoc punctiforme | heptane + formate + H2O + 2 NAD+ | GC-MS poduct analysis | ? |
Synonyms | Comment | Organism |
---|---|---|
ADO | - |
Nostoc punctiforme |
aldehyde-deformylating oxygenase | - |
Nostoc punctiforme |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Nostoc punctiforme |
General Information | Comment | Organism |
---|---|---|
evolution | cyanobacterial aldehyde-deformylating oxygenases belong to the ferritin-like diiron-carboxylate superfamily of dioxygen-activating proteins | Nostoc punctiforme |