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Literature summary for 4.1.99.5 extracted from

  • Zhang, J.; Lu, X.; Li, J.J.
    Conversion of fatty aldehydes into alk (a/e)nes by in vitro reconstituted cyanobacterial aldehyde-deformylating oxygenase with the cognate electron transfer system (2013), Biotechnol. Biofuels, 6, 86.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
cloning of gene Synpcc7942_159 from strain PCC7942, overexpression of N-terminally His-tagged protein in Escherichia coli strain BL21(DE3) Synechococcus elongatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.244
-
n-Heptanal pH 7.2, 22°C, recombinant enzyme with Fd/FNR system Synechococcus elongatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26400
-
x * 26400, recombinant N-terminally His-tagged enzyme, SDS-PAGE Synechococcus elongatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
long-chain aldehyde + O2 + 2 NADPH + 2 H+ Synechococcus elongatus
-
alkane + formate + H2O + 2 NADP+
-
?
long-chain aldehyde + O2 + 2 NADPH + 2 H+ Synechococcus elongatus PCC 7942
-
alkane + formate + H2O + 2 NADP+
-
?

Organism

Organism UniProt Comment Textmining
Synechococcus elongatus Q54764
-
-
Synechococcus elongatus PCC 7942 Q54764
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, removal of the tag by thrombin, and gel filtration Synechococcus elongatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hexadecanal + O2 + 2 NAD(P)H + 2 H+ endogenous reducing system ferredoxin-mediated the cytochrome c reduction with ferredoxin-NADP+ reductase Synechococcus elongatus pentadecane + formate + H2O + 2 NAD(P)+
-
?
hexadecanal + O2 + 2 NAD(P)H + 2 H+ endogenous reducing system ferredoxin-mediated the cytochrome c reduction with ferredoxin-NADP+ reductase Synechococcus elongatus PCC 7942 pentadecane + formate + H2O + 2 NAD(P)+
-
?
long-chain aldehyde + O2 + 2 NADPH + 2 H+
-
Synechococcus elongatus alkane + formate + H2O + 2 NADP+
-
?
long-chain aldehyde + O2 + 2 NADPH + 2 H+
-
Synechococcus elongatus PCC 7942 alkane + formate + H2O + 2 NADP+
-
?
additional information the endogenous electron transfer system works more effectively than the heterologous and chemical ones, e.g. phenazine methosulfate or 1-methyoxy-5-methylphenazinium methylsulfate and NADH, overview Synechococcus elongatus ?
-
?
additional information the endogenous electron transfer system works more effectively than the heterologous and chemical ones, e.g. phenazine methosulfate or 1-methyoxy-5-methylphenazinium methylsulfate and NADH, overview Synechococcus elongatus PCC 7942 ?
-
?
n-heptanal + O2 + 2 NAD(P)H + 2 H+ endogenous reducing system ferredoxin-mediated the cytochrome c reduction with ferredoxin-NADP+ reductase Synechococcus elongatus n-hexane + formate + H2O + 2 NAD(P)+
-
?
n-heptanal + O2 + 2 NAD(P)H + 2 H+ endogenous reducing system ferredoxin-mediated the cytochrome c reduction with ferredoxin-NADP+ reductase Synechococcus elongatus PCC 7942 n-hexane + formate + H2O + 2 NAD(P)+
-
?
octadecanal + O2 + 2 NAD(P)H + 2 H+ endogenous reducing system ferredoxin-mediated the cytochrome c reduction with ferredoxin-NADP+ reductase Synechococcus elongatus heptadecane + formate + H2O + 2 NAD(P)+
-
?
octadecanal + O2 + 2 NAD(P)H + 2 H+ endogenous reducing system ferredoxin-mediated the cytochrome c reduction with ferredoxin-NADP+ reductase Synechococcus elongatus PCC 7942 heptadecane + formate + H2O + 2 NAD(P)+
-
?

Subunits

Subunits Comment Organism
? x * 26400, recombinant N-terminally His-tagged enzyme, SDS-PAGE Synechococcus elongatus

Synonyms

Synonyms Comment Organism
ADO
-
Synechococcus elongatus
aldehyde decarbonylase UniProt Synechococcus elongatus
aldehyde-deformylating oxygenase
-
Synechococcus elongatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at room temperature Synechococcus elongatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0073
-
n-Heptanal pH 7.2, 22°C, recombinant enzyme with Fd/FNR system Synechococcus elongatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
assay at Synechococcus elongatus

Cofactor

Cofactor Comment Organism Structure
additional information ferredoxin-mediated the cytochrome c reduction with ferredoxin-NADP+ reductase: ADO is selective against ferredoxin and the interaction between ferredoxin and ferredoxin-NADP+ reductase is very important for efficient electron transfer and ADO activity Synechococcus elongatus
NADH
-
Synechococcus elongatus
NADPH physiological cofactor Synechococcus elongatus