Cloned (Comment) | Organism |
---|---|
recombinant expression in Escherichia coli | Nostoc punctiforme |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | trans-3-nonyloxirane-2-carbaldehyde and trans-3-pentadecanyloxirane-2-carbaldehyde are no inhibitors of the enzyme | Nostoc punctiforme |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | dependent on | Nostoc punctiforme |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
long-chain aldehyde + O2 + 2 NADPH + 2 H+ | Nostoc punctiforme | - |
alkane + formate + H2O + 2 NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nostoc punctiforme | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli | Nostoc punctiforme |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
octadecanal + O2 + 2 NADPH + 2 H+ = heptadecane + formate + H2O + 2 NADP+ | proposed mechanism of cADO involving homolytic cleavage of the C1-C2 bond of aldehyde by di-iron peroxo species, and proposed mechanism for deformylation involving heterolytic cleavage of the C1-C2 bond | Nostoc punctiforme |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dodecanal + O2 + 2 NADH + 2 H+ | with reducing system NADH/phenazine methosulfate | Nostoc punctiforme | undecane + formate + H2O + 2 NAD+ | - |
? | |
long-chain aldehyde + O2 + 2 NADPH + 2 H+ | - |
Nostoc punctiforme | alkane + formate + H2O + 2 NADP+ | - |
? | |
additional information | the enzyme is more active with either long-chain (C18-C14) or short-chain (C9-C5) aldehydes whereas medium chain aldehydes, including dodecanal, are turned over considerably more slowly | Nostoc punctiforme | ? | - |
? | |
octadecanal + O2 + 2 NADH + 2 H+ | with reducing system NADH/phenazine methosulfate | Nostoc punctiforme | heptadecane + formate + H2O + 2 NAD+ | - |
? | |
trans-3-nonyloxirane-2-carbaldehyde + 2 NADH + 2 H+ | with reducing system NADH/phenazine methosulfate | Nostoc punctiforme | 2-nonyloxirane + formate + H2O + 2 NAD+ | - |
? | |
trans-3-pentadecanyloxirane-2-carbaldehyde + 2 NADH + O2 + 2 H+ | with reducing system NADH/phenazine methosulfate | Nostoc punctiforme | 2-pentadecanyloxirane + formate + 2 NAD+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aldehyde deformylating oxygenase | - |
Nostoc punctiforme |
cADO | - |
Nostoc punctiforme |
cyanobacterial aldehyde decarbonylase | - |
Nostoc punctiforme |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Nostoc punctiforme |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00027 | - |
trans-3-nonyloxirane-2-carbaldehyde | pH 7.2, 37°C | Nostoc punctiforme | |
0.00048 | - |
trans-3-pentadecanyloxirane-2-carbaldehyde | pH 7.2, 37°C | Nostoc punctiforme | |
0.0018 | - |
octadecanal | pH 7.2, 37°C | Nostoc punctiforme |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Nostoc punctiforme |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Nostoc punctiforme | |
NADPH | physiological cofactor | Nostoc punctiforme |
General Information | Comment | Organism |
---|---|---|
metabolism | in cyanobacteria, aldehyde deformylating oxygenase catalyzes the decarbonylation of fatty aldehydes to the corresponding alkanes or alkenes, last step in the biosynthesis of long-chain aliphatic hydrocarbons, which are derived from fatty acids | Nostoc punctiforme |