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Literature summary for 4.1.99.5 extracted from

  • Aukema, K.G.; Makris, T.M.; Stoian, S.A.; Richman, J.E.; Muenck, E.; Lipscomb, J.D.; Wackett, L.P.
    Cyanobacterial aldehyde deformylase oxygenation of aldehydes yields n-1 aldehydes and alcohols in addition to alkanes (2013), ACS Catal., 3, 2228-2238.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Prochlorococcus marinus

Organism

Organism UniProt Comment Textmining
Prochlorococcus marinus Q7V6D4 gene PMT_1231
-
Prochlorococcus marinus MIT 9313 Q7V6D4 gene PMT_1231
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by anion exchange chromatography and dialysis Prochlorococcus marinus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
decanal + O2 + 2 NADH + 2 H+ with reducing system NADH/phenazine Prochlorococcus marinus nonane + formate + H2O + 2 NAD+
-
?
decanal + O2 + 2 NADH + 2 H+ with reducing system NADH/phenazine Prochlorococcus marinus MIT 9313 nonane + formate + H2O + 2 NAD+
-
?
additional information substrate specificity, overview. Marked decrease in relative yields of aldehyde and alcohol products are observed as the alkyl chain length is decreased from C9 to C8. The relative yields of the one-carbon-shorter alcohol and aldehyde products are optimal with nonanal and decanal and decrease with shorter and longer alkyl chains Prochlorococcus marinus ?
-
?
additional information substrate specificity, overview. Marked decrease in relative yields of aldehyde and alcohol products are observed as the alkyl chain length is decreased from C9 to C8. The relative yields of the one-carbon-shorter alcohol and aldehyde products are optimal with nonanal and decanal and decrease with shorter and longer alkyl chains Prochlorococcus marinus MIT 9313 ?
-
?
nonanal + O2 + 2 NADH + 2 H+ with reducing system NADH/phenazine Prochlorococcus marinus octane + formate + H2O + 2 NAD+
-
?
nonanal + O2 + 2 NADH + 2 H+ with reducing system NADH/phenazine Prochlorococcus marinus MIT 9313 octane + formate + H2O + 2 NAD+
-
?
octadecanal + O2 + 2 NADH + 2 H+ with reducing system NADH/phenazine Prochlorococcus marinus heptadecane + formate + H2O + 2 NAD+
-
?
octadecanal + O2 + 2 NADH + 2 H+ with reducing system NADH/phenazine Prochlorococcus marinus MIT 9313 heptadecane + formate + H2O + 2 NAD+
-
?

Synonyms

Synonyms Comment Organism
ADO
-
Prochlorococcus marinus
aldehyde deformylase
-
Prochlorococcus marinus
aldehyde-deformylating oxygenase
-
Prochlorococcus marinus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at room temperature Prochlorococcus marinus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Prochlorococcus marinus

Cofactor

Cofactor Comment Organism Structure
NADH
-
Prochlorococcus marinus