Cloned (Comment) | Organism |
---|---|
expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) | Prochlorococcus marinus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Prochlorococcus marinus | Q7V6D4 | gene PMT_1231 | - |
Prochlorococcus marinus MIT 9313 | Q7V6D4 | gene PMT_1231 | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by anion exchange chromatography and dialysis | Prochlorococcus marinus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
decanal + O2 + 2 NADH + 2 H+ | with reducing system NADH/phenazine | Prochlorococcus marinus | nonane + formate + H2O + 2 NAD+ | - |
? | |
decanal + O2 + 2 NADH + 2 H+ | with reducing system NADH/phenazine | Prochlorococcus marinus MIT 9313 | nonane + formate + H2O + 2 NAD+ | - |
? | |
additional information | substrate specificity, overview. Marked decrease in relative yields of aldehyde and alcohol products are observed as the alkyl chain length is decreased from C9 to C8. The relative yields of the one-carbon-shorter alcohol and aldehyde products are optimal with nonanal and decanal and decrease with shorter and longer alkyl chains | Prochlorococcus marinus | ? | - |
? | |
additional information | substrate specificity, overview. Marked decrease in relative yields of aldehyde and alcohol products are observed as the alkyl chain length is decreased from C9 to C8. The relative yields of the one-carbon-shorter alcohol and aldehyde products are optimal with nonanal and decanal and decrease with shorter and longer alkyl chains | Prochlorococcus marinus MIT 9313 | ? | - |
? | |
nonanal + O2 + 2 NADH + 2 H+ | with reducing system NADH/phenazine | Prochlorococcus marinus | octane + formate + H2O + 2 NAD+ | - |
? | |
nonanal + O2 + 2 NADH + 2 H+ | with reducing system NADH/phenazine | Prochlorococcus marinus MIT 9313 | octane + formate + H2O + 2 NAD+ | - |
? | |
octadecanal + O2 + 2 NADH + 2 H+ | with reducing system NADH/phenazine | Prochlorococcus marinus | heptadecane + formate + H2O + 2 NAD+ | - |
? | |
octadecanal + O2 + 2 NADH + 2 H+ | with reducing system NADH/phenazine | Prochlorococcus marinus MIT 9313 | heptadecane + formate + H2O + 2 NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADO | - |
Prochlorococcus marinus |
aldehyde deformylase | - |
Prochlorococcus marinus |
aldehyde-deformylating oxygenase | - |
Prochlorococcus marinus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Prochlorococcus marinus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Prochlorococcus marinus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Prochlorococcus marinus |