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Literature summary for 4.1.99.3 extracted from
- Identification and Purification of the CPD Photolyase in Vibrio parahaemolyticus RIMD2210633 (2015), Photochem. Photobiol., 91, 1165-1172 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene VPA1471 or phr, DNA and amino acid sequence determination and analysis, quantitative real-time PCR expression analysis, functional recombinant expression of His6-tagged enzyme in Escherichia coli from pET-30a vector | Vibrio parahaemolyticus RIMD 2210633 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cyclobutadipyrimidine (in DNA) | Vibrio parahaemolyticus RIMD 2210633 | - |
2 pyrimidine residues (in DNA) | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vibrio parahaemolyticus RIMD 2210633 | Q87G46 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme from Escherichia coli | Vibrio parahaemolyticus RIMD 2210633 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cyclobutadipyrimidine (in DNA) | - |
Vibrio parahaemolyticus RIMD 2210633 | 2 pyrimidine residues (in DNA) | - |
? | |
| additional information | usage of salmon sperm DNA with introduced CPDs (UVC-irradiation) as assay substrate | Vibrio parahaemolyticus RIMD 2210633 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 57000, recombinant His6-tagged enzyme, SDS-PAGE | Vibrio parahaemolyticus RIMD 2210633 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CPD photolyase | - |
Vibrio parahaemolyticus RIMD 2210633 |
| deoxyribodipyrimidine photolyase | - |
Vibrio parahaemolyticus RIMD 2210633 |
| PHR | - |
Vibrio parahaemolyticus RIMD 2210633 |
| VPA1471 | - |
Vibrio parahaemolyticus RIMD 2210633 |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Vibrio parahaemolyticus RIMD 2210633 | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Vibrio parahaemolyticus RIMD 2210633 | the VPA1471 gene expression level increases during UVA illumination following UVC irradiation | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the cryptochrome/photolyase family (CPF) | Vibrio parahaemolyticus RIMD 2210633 |
| physiological function | UV-induced lesions can be repaired by enzymes called photolyases. DNA photolyases use blue/near-UV light to remove these lesions using a process referred to as photoreactivation. CPD photolyases specifically repair CPD lesions in DNA | Vibrio parahaemolyticus RIMD 2210633 |
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