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Literature summary for 4.1.99.3 extracted from

  • Chang, C.W.; Guo, L.; Kao, Y.T.; Li, J.; Tan, C.; Li, T.; Saxena, C.; Liu, Z.; Wang, L.; Sancar, A.; Zhong, D.
    Ultrafast solvation dynamics at binding and active sites of photolyases (2010), Proc. Natl. Acad. Sci. USA, 107, 2914-2919.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
N341A life-time of 2.9 ns compared to 1.3 ns for the wild-type enzyme Escherichia coli
W395R fully binds methenyltetrahydrofolate but not fully reduced FADH-, life-time of 2.9 ns Caulobacter vibrioides

General Stability

General Stability Organism
life-time of wild-type enzyme is 1.3 ns Escherichia coli

Organism

Organism UniProt Comment Textmining
Caulobacter vibrioides
-
-
-
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Caulobacter vibrioides
purified with neutral radical FADH and/or oxidized FAD forms and then converted to a fully reduced FADH- form under anaerobic conditions Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyclobutadipyrimidine in DNA
-
Caulobacter vibrioides ?
-
?
cyclobutadipyrimidine in DNA presence of a very rigid antenna binding site, a relatively rigid active site in CPD photolyase but with large local orientation flexibility Escherichia coli ?
-
?

Synonyms

Synonyms Comment Organism
CPD photolyase
-
Escherichia coli
CPD photolyase
-
Caulobacter vibrioides
PHR
-
Escherichia coli
PHR
-
Caulobacter vibrioides

Cofactor

Cofactor Comment Organism Structure
FADH2
-
Caulobacter vibrioides
FADH2 heterogeneous dynamics continuously tune local configurations to optimize photolyase's function through resonance energy transfer from the antenna to the cofactor for energy efficiency and then electron transfer between the cofactor and the substrate for repair of damaged DNA Escherichia coli
methenyltetrahydrofolate
-
Escherichia coli
methenyltetrahydrofolate
-
Caulobacter vibrioides