Cloned (Comment) | Organism |
---|---|
expressed in a photolyase-deficient Escherichia coli strains NKJ3002 and KY20 | Glycine max |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
85000 | - |
SDS-PAGE | Glycine max |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | B1B538 | - |
- |
Purification (Comment) | Organism |
---|---|
partial purification, glutathione-Sepharose column chromatography | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyclobutadipyrimidine in DNA | - |
Glycine max | pyrimidine residues in DNA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 85000, SDS-PAGE | Glycine max |
Synonyms | Comment | Organism |
---|---|---|
CDP photolyase | class II CPD photolyase | Glycine max |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 45 | when CDP photolyase is heat-treated in vitro from 25 to 45°C for 3 min, thymine dimer-binding activity and photoreactivation activity are decreased and FAD is released from the enzyme, on the other hand photoreactivation activity is stable when the enzyme-CDP complex is heat-treated from 25 to 45°C, the enzyme alone is unstable but once it binds to a substrate the enzyme becomes thermostable | Glycine max |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Glycine max |