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Literature summary for 4.1.99.3 extracted from

  • Yamamoto, A.; Tanbir, N.; Hirouchi, T.; Teranishi, M.; Hidema, J.; Morioka, H.; Yamamoto, K.
    Temperature-sensitive photoreactivation of cyclobutane thymine dimer in soybean (2008), J. Radiat. Res., 49, 189-196.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in a photolyase-deficient Escherichia coli strains NKJ3002 and KY20 Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
85000
-
SDS-PAGE Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max B1B538
-
-

Purification (Commentary)

Purification (Comment) Organism
partial purification, glutathione-Sepharose column chromatography Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyclobutadipyrimidine in DNA
-
Glycine max pyrimidine residues in DNA
-
?

Subunits

Subunits Comment Organism
? x * 85000, SDS-PAGE Glycine max

Synonyms

Synonyms Comment Organism
CDP photolyase class II CPD photolyase Glycine max

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 45 when CDP photolyase is heat-treated in vitro from 25 to 45°C for 3 min, thymine dimer-binding activity and photoreactivation activity are decreased and FAD is released from the enzyme, on the other hand photoreactivation activity is stable when the enzyme-CDP complex is heat-treated from 25 to 45°C, the enzyme alone is unstable but once it binds to a substrate the enzyme becomes thermostable Glycine max

Cofactor

Cofactor Comment Organism Structure
FAD
-
Glycine max