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Literature summary for 4.1.99.3 extracted from
- Influence of ultraviolet-C on structure and function of Synechococcus sp. PCC 7942 photolyase (2007), Biochemistry (Moscow), 72, 540-544.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloned in Escherichia coli | Synechococcus elongatus PCC 7942 = FACHB-805 |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
SDS-PAGE | Synechococcus elongatus PCC 7942 = FACHB-805 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Synechococcus elongatus PCC 7942 = FACHB-805 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| protein is purified with a Ni-chelating HP column | Synechococcus elongatus PCC 7942 = FACHB-805 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
photoreactivation experiments indicate that the survival rate decreases from 100 to 2.6% when the UV-C flux is increased from 1.1 to 68.5 microWatt/cm2. Photolyase is inactivated at UV-C intensities = 25.5 microWatt/cm2 due to structure changes of the photolyase. | Synechococcus elongatus PCC 7942 = FACHB-805 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cyclobutadipyrimidine in DNA | - |
Synechococcus elongatus PCC 7942 = FACHB-805 | 2 pyrimidine residues in DNA | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | spectrometric analysis indicate that tertiary structure of the photolyase changes evidently when the UV-C fluxes are = 25.5 microWatt/cm2, while the secondary structure is almost unchanged even at 170 microWatt/cm2 | Synechococcus elongatus PCC 7942 = FACHB-805 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| photolyase | - |
Synechococcus elongatus PCC 7942 = FACHB-805 |
| PHR | - |
Synechococcus elongatus PCC 7942 = FACHB-805 |
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