Literature summary for 4.1.99.3 extracted from

Byrdin, M.; Villette, S.; Eker, A.P.; Brettel, K.
Observation of an intermediate tryptophanyl radical in W306F mutant DNA photolyase from Escherichia coli supports electron hopping along the triple tryptophan chain (2007), Biochemistry, 46, 10072-10077.

Search for more literature for 4.1.99.3

Cloned(Commentary)

Cloned (Comment)	Organism
cloned in Escherichia coli	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P00914	-	-

Purification (Commentary)

Purification (Comment)	Organism
cells are destructed by sonication in the presence of 10 mM 2-mercaptoethanol. After centrifugation photolyase is purified by chromatography on heparin-Sepharose C1-6B resin by elution with a 0.1 to 1.1 M NaCl gradient in 0.01 M potassium phosphate (pH 7.0) and 10 mM mercaptoethanol, followed by chromatography on SP-Sepharose fast flow resin eluted with a 0.04 to 0.3 M NaCl gradient	Escherichia coli

Reaction

Reaction	Comment	Organism	Reaction ID
cyclobutadipyrimidine (in DNA) = 2 pyrimidine residues (in DNA)	results support the electron hopping mechanism by which electron transfer from W306 to the flavin is mediated in a three-step electron hopping process (W306 to W359 to W382 to FADH)	Escherichia coli	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cyclobutadipyrimidine in DNA	-	Escherichia coli	2 pyrimidine residues in DNA	-	?

Synonyms

Synonyms	Comment	Organism
photolyase	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Escherichia coli

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Release 2023.1 (January 2023)