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Literature summary for 4.1.99.3 extracted from

  • Sutherland, B.M.
    Photoreactivating enzyme from human leukocytes (1974), Nature, 248, 109-112.
    View publication on PubMed

Application

Application Comment Organism
analysis the enzyme is of primary biological interest as an analytical tool Homo sapiens

General Stability

General Stability Organism
treatment of the enzyme with 10 mg trypsin for 1 and 5 min at 34°C decreases enzyme activity to 85% and 59% of its original activity Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
leukocyte
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyclobutadipyrimidine in DNA
-
Homo sapiens pyrimidine residues in DNA
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6.8 8.1 pH 6.8: 49% of maximal activity, pH 8.1: about 29% of maximal activity Homo sapiens