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Literature summary for 4.1.99.22 extracted from

  • Hover, B.M.; Yokoyama, K.
    C-Terminal glycine-gated radical initiation by GTP 3',8-cyclase in the molybdenum cofactor biosynthesis (2015), J. Am. Chem. Soc., 137, 3352-3359.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.99.22

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Staphylococcus aureus

Protein Variants

Protein Variants Comment Organism
D198A mutation in GG motif, decrease in affinity for S-adenosine-L-methionine Staphylococcus aureus
G339A mutation in GG motif, loss of activity Staphylococcus aureus
G340A mutation in GG motif, loss of activity Staphylococcus aureus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0031
-
 GTP wild-type, pH 7.6, 25°C Staphylococcus aureus
0.0051
-
 S-adenosyl-L-methionine wild-type, pH 7.6, 25°C Staphylococcus aureus
0.0069
-
 GTP mutant D198A, pH 7.6, 25°C Staphylococcus aureus
0.036
-
 S-adenosyl-L-methionine mutant D198A, pH 7.6, 25°C Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + S-adenosyl-L-methionine + reduced electron acceptor Staphylococcus aureus
-
 (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + oxidized electron acceptor
-
 ?
GTP + S-adenosyl-L-methionine + reduced electron acceptor Staphylococcus aureus N315
-
 (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + oxidized electron acceptor
-
 ?

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus P65388
-
-
Staphylococcus aureus N315 P65388
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + S-adenosyl-L-methionine + reduced electron acceptor
-
 Staphylococcus aureus (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + oxidized electron acceptor
-
 ?
GTP + S-adenosyl-L-methionine + reduced electron acceptor
-
 Staphylococcus aureus N315 (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + oxidized electron acceptor
-
 ?

Synonyms

Synonyms Comment Organism
MoaA
-
 Staphylococcus aureus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00007
-
 S-adenosyl-L-methionine mutant D198A, pH 7.6, 25°C Staphylococcus aureus
0.00008
-
 GTP mutant D198A, pH 7.6, 25°C Staphylococcus aureus
0.0007
-
 GTP wild-type, pH 7.6, 25°C Staphylococcus aureus
0.00075
-
 S-adenosyl-L-methionine wild-type, pH 7.6, 25°C Staphylococcus aureus

General Information

General Information Comment Organism
physiological function the GG motif is essential for the activity of MoaA to produce (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate from GTP, and synthetic peptides corresponding to the C-terminal region of wild-type MoaA rescue the GTP 3',8-cyclase activity of the GG-motif mutants. The C-terminal tail containing the GG motif interacts with the SAM-binding pocket of MoaA, and is essential for the binding of SAM and subsequent radical initiation Staphylococcus aureus