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Literature summary for 4.1.99.22 extracted from
- Crystal structure of the S-adenosylmethionine-dependent enzyme MoaA and its implications for molybdenum cofactor deficiency in humans (2004), Proc. Natl. Acad. Sci. USA, 101, 12870-12875.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| MoaC protein | formation cyclic pyranopterin monophosphate by MoaA requires the action of MoaC protein | Staphylococcus aureus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed as His-tagged proteins in Escherichia coli | Staphylococcus aureus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| crystals are grown under anaerobic conditions, hanging drop vapor diffusion technique, crystals belong to space group P2(1)2(1)2(1) with cell dimensions of a = 48.1, b = 102.4, and c = 191.2 A and contain two molecules per asymmetric unit, structures of MoaA in the apo-state (2.8 A) and in complex with S-adenosyl-L-methionine (2.2 A) | Staphylococcus aureus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| iron-sulfur centre | binds 1 4Fe-4S cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 4Fe-4S cluster coordinated with 3 cysteines and the GTP-derived substrate | Staphylococcus aureus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41000 | - |
monomer, in solution MoaA exists as a monomer (41000 Da) and dimer (82000 Da), gel filtration | Staphylococcus aureus |
| 42000 | - |
1 * 42000, in solution MoaA exists as a monomer (41000 Da) and dimer (82000 Da) | Staphylococcus aureus |
| 42000 | - |
2 * 42000, in solution MoaA exists as a monomer (41000 Da) and dimer (82000 Da) | Staphylococcus aureus |
| 82000 | - |
homodimer, in solution MoaA exists as a monomer (41000 Da) and dimer (82000 Da), gel filtration | Staphylococcus aureus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP | Staphylococcus aureus | the enzyme catalyses an early step in the biosynthesis of the molybdenum cofactor | cyclic pyranopterin phosphate + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus aureus | P65388 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purified under anaerobic conditions | Staphylococcus aureus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP | the enzyme catalyses an early step in the biosynthesis of the molybdenum cofactor | Staphylococcus aureus | cyclic pyranopterin phosphate + diphosphate | - |
? | |
| GTP | the reaction is catalysed by MoaA and requires the action of MoaC | Staphylococcus aureus | cyclic pyranopterin phosphate + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 42000, in solution MoaA exists as a monomer (41000 Da) and dimer (82000 Da) | Staphylococcus aureus |
| monomer | 1 * 42000, in solution MoaA exists as a monomer (41000 Da) and dimer (82000 Da) | Staphylococcus aureus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MoaA | - |
Staphylococcus aureus |
| MoaC | - |
Staphylococcus aureus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| iron-sulfur centre | binds 1 4Fe-4S cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 4Fe-4S cluster coordinated with 3 cysteines and the GTP-derived substrate | Staphylococcus aureus | |
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