Activating Compound | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent on, activates | Fusobacterium nucleatum subsp. nucleatum |
Application | Comment | Organism |
---|---|---|
synthesis | high activity and high level expression of Fn-TPL in Escherichia coli provides a cheap and robust biocatalyst for large-scale production of L-DOPA | Fusobacterium nucleatum subsp. nucleatum |
Cloned (Comment) | Organism |
---|---|
gene tnaA, recombinant expression of codon-optimized His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Fusobacterium nucleatum subsp. nucleatum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
L-tyrosine | recombinant enzyme, pH 8.5, 20°C | Fusobacterium nucleatum subsp. nucleatum | |
3.6 | - |
phenol | recombinant enzyme, L-tyrosine formation is measured from phenol, pyruvate, and ammonium, pH 8.5, 20°C | Fusobacterium nucleatum subsp. nucleatum | |
6.28 | - |
L-Dopa | recombinant enzyme, pH 8.5, 20°C | Fusobacterium nucleatum subsp. nucleatum | |
15.62 | - |
pyrocatechol | recombinant enzyme, L-DOPA formation is measured from dicatechol, pyruvate, and ammonium, pH 8.5, 20°C | Fusobacterium nucleatum subsp. nucleatum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine + H2O | Fusobacterium nucleatum subsp. nucleatum | L-tyrosine is the physiological substrate of enzyme Fn-TPL | phenol + pyruvate + NH3 | - |
r | |
L-tyrosine + H2O | Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131 | L-tyrosine is the physiological substrate of enzyme Fn-TPL | phenol + pyruvate + NH3 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fusobacterium nucleatum subsp. nucleatum | Q8RHM6 | - |
- |
Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131 | Q8RHM6 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis | Fusobacterium nucleatum subsp. nucleatum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cysteine + H2O | 12% of the activity with L-tyrosine | Fusobacterium nucleatum subsp. nucleatum | thiophenol + pyruvate + NH3 | - |
r | |
L-DOPA + H2O | 42% of the activity with L-tyrosine | Fusobacterium nucleatum subsp. nucleatum | pyrocatechol + pyruvate + NH4+ | - |
r | |
L-phenylalanine + H2O | 7% of the activity with L-tyrosine | Fusobacterium nucleatum subsp. nucleatum | ? + pyruvate + NH3 | - |
r | |
L-serine + H2O | 9% of the activity with L-tyrosine | Fusobacterium nucleatum subsp. nucleatum | pyruvate + NH3 | - |
r | |
L-tryptophan + H2O | 8% of the activity with L-tyrosine, reaction of EC 4.1.99.1 | Fusobacterium nucleatum subsp. nucleatum | indole + pyruvate + NH3 | - |
r | |
L-tryptophan + H2O | 8% of the activity with L-tyrosine, reaction of EC 4.1.99.1 | Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131 | indole + pyruvate + NH3 | - |
r | |
L-tyrosine + H2O | L-tyrosine is the physiological substrate of enzyme Fn-TPL | Fusobacterium nucleatum subsp. nucleatum | phenol + pyruvate + NH3 | - |
r | |
L-tyrosine + H2O | alpha,beta-elimination of L-tyrosine, best substrate | Fusobacterium nucleatum subsp. nucleatum | phenol + pyruvate + NH3 | - |
r | |
L-tyrosine + H2O | L-tyrosine is the physiological substrate of enzyme Fn-TPL | Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131 | phenol + pyruvate + NH3 | - |
r | |
L-tyrosine + H2O | alpha,beta-elimination of L-tyrosine, best substrate | Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131 | phenol + pyruvate + NH3 | - |
r | |
L-valine + H2O | 6% of the activity with L-tyrosine | Fusobacterium nucleatum subsp. nucleatum | ? + pyruvate + NH3 | - |
r | |
additional information | low activity of Fn-TPL is observed toward natural amino acids including L-serine, L-cysteine, L-phenylalanine, L-valine, L-tryptophan, no activity with L-threonine, L-glutamine, L-alanine, D-alanine, L-arginine, L-leucine, L-histidine, L-aspartic acid, L-methionine, and D-methionine | Fusobacterium nucleatum subsp. nucleatum | ? | - |
? | |
additional information | low activity of Fn-TPL is observed toward natural amino acids including L-serine, L-cysteine, L-phenylalanine, L-valine, L-tryptophan, no activity with L-threonine, L-glutamine, L-alanine, D-alanine, L-arginine, L-leucine, L-histidine, L-aspartic acid, L-methionine, and D-methionine | Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131 | ? | - |
? | |
phenol + pyruvate + NH3 | - |
Fusobacterium nucleatum subsp. nucleatum | L-tyrosine + H2O | - |
r | |
pyrocatechol + pyruvate + NH4+ | - |
Fusobacterium nucleatum subsp. nucleatum | L-DOPA + H2O | - |
r | |
pyrocatechol + pyruvate + NH4+ | - |
Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131 | L-DOPA + H2O | - |
r | |
S-methyl-L-cysteine + H2O | 13% of the activity with L-tyrosine | Fusobacterium nucleatum subsp. nucleatum | methanthiol + pyruvate + NH3 | - |
r |
Synonyms | Comment | Organism |
---|---|---|
Fn-TPL | - |
Fusobacterium nucleatum subsp. nucleatum |
TnaA | - |
Fusobacterium nucleatum subsp. nucleatum |
TPL | - |
Fusobacterium nucleatum subsp. nucleatum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Fusobacterium nucleatum subsp. nucleatum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 80 | activity range, profile overview | Fusobacterium nucleatum subsp. nucleatum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.44 | - |
phenol | recombinant enzyme, L-tyrosine formation is measured from phenol, pyruvate, and ammonium, pH 8.5, 20°C | Fusobacterium nucleatum subsp. nucleatum | |
0.54 | - |
L-Dopa | recombinant enzyme, pH 8.5, 20°C | Fusobacterium nucleatum subsp. nucleatum | |
1.72 | - |
L-tyrosine | recombinant enzyme, pH 8.5, 20°C | Fusobacterium nucleatum subsp. nucleatum | |
2.68 | - |
pyrocatechol | recombinant enzyme, L-DOPA formation is measured from dicatechol, pyruvate, and ammonium, pH 8.5, 20°C | Fusobacterium nucleatum subsp. nucleatum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Fusobacterium nucleatum subsp. nucleatum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10 | activity range, profile overview | Fusobacterium nucleatum subsp. nucleatum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent on, activates, Km value is 0.0034 mM | Fusobacterium nucleatum subsp. nucleatum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
L-Dopa | recombinant enzyme, pH 8.5, 20°C | Fusobacterium nucleatum subsp. nucleatum | |
0.12 | - |
phenol | recombinant enzyme, L-tyrosine formation is measured from phenol, pyruvate, and ammonium, pH 8.5, 20°C | Fusobacterium nucleatum subsp. nucleatum | |
9.58 | - |
L-tyrosine | recombinant enzyme, pH 8.5, 20°C | Fusobacterium nucleatum subsp. nucleatum |