Protein Variants | Comment | Organism |
---|---|---|
H343A | all substrates for alpha,beta-elimination, except S-ethyl-L-Cys, exhibit lower turnover number values with the mutant enzyme than with the wild-type enzyme. The mutant shows slower rates of deuterium isotope exchange for L-Phe and L-Met than does the wild type enzyme. The turnover-number for 3-fluoro-L-Tyr is pH-dependent for the mutant enzyme, whereas it is pH-independent for the wild-type enzyme. His343 does play an important function in catalysis, possibly by facilitating the conformational change from an open' to closed' form when substrates bind | Citrobacter freundii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
S-(o-nitrophenyl)-L-Cys | wild-type enzyme | Citrobacter freundii | |
0.1 | - |
3-fluoro-L-Tyr | wild-type enzyme | Citrobacter freundii | |
0.2 | - |
L-Tyr | wild-type enzyme | Citrobacter freundii | |
0.2 | - |
S-benzyl-L-Cys | wild-type enzyme | Citrobacter freundii | |
0.5 | - |
S-(o-nitrophenyl)-L-Cys | mutant enzyme H343A | Citrobacter freundii | |
1.2 | - |
S-ethyl-L-Cys | mutant enzyme H343A | Citrobacter freundii | |
1.2 | - |
S-benzyl-L-Cys | mutant enzyme H343A | Citrobacter freundii | |
1.2 | - |
3-fluoro-L-Tyr | mutant enzyme H343A | Citrobacter freundii | |
2.2 | - |
L-Tyr | mutant enzyme H343A | Citrobacter freundii | |
3.4 | - |
S-methyl-L-Cys | wild-type enzyme | Citrobacter freundii | |
6.6 | - |
S-ethyl-L-Cys | wild-type enzyme | Citrobacter freundii | |
11 | - |
L-Ala | wild-type enzyme | Citrobacter freundii | |
11 | - |
L-Ser | mutant enzyme H343A | Citrobacter freundii | |
12.99 | - |
S-methyl-L-Cys | mutant enzyme H343A | Citrobacter freundii | |
18 | - |
L-Ser | wild-type enzyme | Citrobacter freundii | |
32 | - |
D-Ala | wild-type enzyme, alpha,beta-elimination | Citrobacter freundii | |
56.4 | - |
S-methyl-L-Cys | wild-type enzyme | Citrobacter freundii | |
103 | - |
L-Ala | mutant enzyme H343A | Citrobacter freundii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrobacter freundii | - |
wild type enzyme and mutant enzyme H343A | - |
Purification (Comment) | Organism |
---|---|
mutant enzyme His343Ala | Citrobacter freundii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.9 | - |
- |
Citrobacter freundii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala + H2O | L-Ala | Citrobacter freundii | ? | - |
? | |
Ala + H2O | D-Ala | Citrobacter freundii | ? | - |
? | |
D-Ala + H2O | - |
Citrobacter freundii | ? | - |
? | |
S-(o-nitrophenyl)-L-Cys + H2O | - |
Citrobacter freundii | ? | - |
? | |
S-benzoyl-L-Cys + H2O | - |
Citrobacter freundii | ? | - |
? | |
S-benzyl-L-Cys + H2O | - |
Citrobacter freundii | ? + pyruvate + NH3 | - |
? | |
S-ethyl-L-Cys + H2O | - |
Citrobacter freundii | ? | - |
? | |
S-methyl-L-Cys + H2O | - |
Citrobacter freundii | ? + pyruvate + NH3 | - |
? | |
Ser + H2O | L-Ser | Citrobacter freundii | ? + pyruvate + NH3 | - |
? | |
Tyr + H2O | - |
Citrobacter freundii | phenol + pyruvate + NH3 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
D-Ala | mutant enzyme H343A | Citrobacter freundii | |
0.01 | - |
D-Ala | wild-type enzyme | Citrobacter freundii | |
0.02 | - |
S-benzoyl-L-Cys | mutant enzyme H343A | Citrobacter freundii | |
0.03 | - |
L-Ala | wild-type enzyme | Citrobacter freundii | |
0.07 | - |
L-Ser | mutant enzyme H343A | Citrobacter freundii | |
0.15 | - |
S-methyl-L-Cys | mutant enzyme H343A | Citrobacter freundii | |
0.17 | - |
L-Ser | wild-type enzyme | Citrobacter freundii | |
0.18 | - |
3-fluoro-L-Tyr | mutant enzyme H343A | Citrobacter freundii | |
0.42 | - |
L-Tyr | mutant enzyme H343A | Citrobacter freundii | |
0.52 | - |
S-benzyl-L-Cys | wild-type enzyme | Citrobacter freundii | |
1.4 | - |
3-fluoro-L-Tyr | wild-type enzyme | Citrobacter freundii | |
1.4 | - |
S-ethyl-L-Cys | mutant enzyme H343A | Citrobacter freundii | |
1.55 | - |
S-(o-nitrophenyl)-L-Cys | mutant enzyme H343A | Citrobacter freundii | |
3.5 | - |
L-Tyr | wild-type enzyme | Citrobacter freundii | |
3.88 | - |
S-ethyl-L-Cys | wild-type enzyme | Citrobacter freundii | |
5.1 | - |
S-(o-nitrophenyl)-L-Cys | wild-type enzyme | Citrobacter freundii |