Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | iron content is 5.2 mol eq of iron per mol of ThiH, contains an 4F-4S cluster essential for activity | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P30140 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-tyrosine + S-adenosyl-L-methionine + NADPH = 2-iminoacetate + 4-methylphenol + 5'-deoxyadenosine + L-methionine + NADP+ + H+ | ThiH is a member of the radical-S-adenosyl-L-methionine family. Proposed mechanism of ThiH-dependent cleavage of L-tyrosine: S-adenosyl-L-methionine is reductively cleaved to yield a highly reactive 5'-deoxyadenosyl radical. This radical is proposed to abstract the phenolic hydrogen atom from tyrosine, and the resultant substrate radical cleaves to yield dehydroglycine, which is required by ThiG for the thiazole cyclization reaction | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
thiH | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
[4Fe-4S]-center | essential for activity | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is essential for thiazole biosynthesis in Escherichia coli | Escherichia coli |