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Literature summary for 4.1.99.19 extracted from

  • Leonardi, R.; Fairhurst, S.A.; Kriek, M.; Lowe, D.J.; Roach, P.L.
    Thiamine biosynthesis in Escherichia coli: isolation and initial characterisation of the ThiGH complex (2003), FEBS Lett., 539, 95-99.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
C-terminally hexahistidine-tagged ThiH(ThiH-His) is expressed in Escherichia coli as a soluble protein from thiGHHis-tag and thiFSGH-His-tag-bearing plasmids Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
4Fe-4S-center
-
Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
the 440000 Da complex contains both ThiG and ThiH-His Escherichia coli
44143
-
x * 44143, calculated from sequence Escherichia coli
44143
-
x * 44143, electrospray mass spectrometry Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P30140
-
-

Purification (Commentary)

Purification (Comment) Organism
when isolated under anaerobic conditions, ThiG and ThiH-His co-purify as a large multimeric non-covalent complex Escherichia coli

Subunits

Subunits Comment Organism
? x * 44143, calculated from sequence Escherichia coli
? x * 44143, electrospray mass spectrometry Escherichia coli

Synonyms

Synonyms Comment Organism
thiH
-
Escherichia coli

General Information

General Information Comment Organism
physiological function involved in the biosynthesis of the thiazole moiety of thiamine Escherichia coli