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Literature summary for 4.1.99.14 extracted from

  • Yang, L.; Lin, G.; Liu, D.; Dria, K.J.; Telser, J.; Li, L.
    Probing the reaction mechanism of spore photoproduct lyase (SPL) via diastereoselectively labeled dinucleotide SP TpT substrates (2011), J. Am. Chem. Soc., 133, 10434-10447.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.99.14

Cloned(Commentary)

Cloned (Comment) Organism
-
 Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
4Fe-4S
-
 Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 SDS-PAGE Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-thyminyl-5,6 dihydrothymine + S-adenosyl-L-methionine via organic synthesis and DNA photochemistry, the two H-atoms at the C6 carbon (6-HproS or 6-HproR position) are selectively labeled with a deuterium in a dinucleotide spore photoproduct TpT substrate. Monitoring the deuterium migration in enzyme catalysis reveals that it is the 6-HproR atom of spore photoproduct that is abstracted by the 5'-dA radical. The abstracted deuterium is not returned to the resulting TpT after enzymatic catalysis, an H-atom from the aqueous buffer is incorporated into TpT instead Bacillus subtilis thymidylyl-(3'-5')-thymidylate + 5'-deoxyadenosine + L-methionine
-
 ?

Synonyms

Synonyms Comment Organism
SPL
-
 Bacillus subtilis
spore photoproduct lyase
-
 Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Bacillus subtilis