Literature summary for 4.1.99.13 extracted from

An, M.; Zheng, Z.; Qu, C.; Wang, X.; Chen, H.; Shi, C.; Miao, J.
The first (6-4) photolyase with DNA damage repair activity from the Antarctic microalga Chlamydomonas sp. ICE-L (2018), Mutat. Res., 809, 13-19 .

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Cloned(Commentary)

Cloned (Comment)	Organism
gene 6-4CiPhr, transcriptome analysis and cloning, DNA and amino acid sequence determination and analysis, phylogenetic tree, real-time PCR enzyme expression analysis, functional recombinant expression of C-terminally His-tagged enzyme in a photolyase-deficient Escherichia coli strain	Chlamydomonas sp. ICE-L

Protein Variants

Protein Variants	Comment	Organism
additional information	expressing 6-4CiPhr in a photolyase-deficient Escherichia coli strain improves survival rate of the strain	Chlamydomonas sp. ICE-L

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(6-4) photoproduct (in DNA)	Chlamydomonas sp. ICE-L	-	2 pyrimidine residues (in DNA)	-	?

Organism

Organism	UniProt	Comment	Textmining
Chlamydomonas sp. ICE-L	A0A2Z5J5J9	isolated from the floating ice near the Zhongshan Research Station of Antarctica	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(6-4) photoproduct (in DNA)	-	Chlamydomonas sp. ICE-L	2 pyrimidine residues (in DNA)	-	?

Subunits

Subunits	Comment	Organism
?	x * 64200, about, sequence calculation	Chlamydomonas sp. ICE-L

Synonyms

Synonyms	Comment	Organism
(6-4) photolyase	-	Chlamydomonas sp. ICE-L
6-4CiPhr	-	Chlamydomonas sp. ICE-L

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
15	-	assay at	Chlamydomonas sp. ICE-L

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.6	-	assay at	Chlamydomonas sp. ICE-L

pI Value

Organism	Comment	pI Value Maximum	pI Value
Chlamydomonas sp. ICE-L	sequence calculation	-	8.86

Expression

Organism	Comment	Expression
Chlamydomonas sp. ICE-L	under UVB exposure, transcription of 6-4CiPhr is upregulated continuously within 6 h, achieving a maximum of 62.7fold at 6 h	up

General Information

General Information	Comment	Organism
physiological function	psychrophilic microalga, Chlamydomonas sp. ICE-L, isolated from floating ice in the Antarctic, one of the most highly UV exposed ecosystems on Earth, displays an efficient DNA photorepair capacity, enzyme 6-4CiPhr is a photolyase with 6-4PP repair activity	Chlamydomonas sp. ICE-L

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Release 2023.1 (January 2023)