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Literature summary for 4.1.99.13 extracted from

  • Zhang, F.; Xu, H.; Cao, Y.; Wen, T.; Lin, J.; Ma, G.; Qiao, D.; Cao, Y.
    Role of Lys281 in the Dunaliella salina (6-4) photolyase reaction (2011), Curr. Microbiol., 62, 146-151.
    View publication on PubMed
Search for more literature for 4.1.99.13

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Dunaliella salina

Protein Variants

Protein Variants Comment Organism
K281G a single T(6-4)T photoproduct in a 10-mer oligonucleotide is photoreactivated by this mutant. Mutant shows similar capacity of photoreactivation compared to wild-type. Over the pH range 6-9 no difference to wild-type. Over the pH range 9-11 the activity of K281G mutant declines sharply and less than 30% of the activity is retained at pH 11.0 Dunaliella salina
K281R a single T(6-4)T photoproduct in a 10-mer oligonucleotide is photoreactivated by this mutant. Mutant shows similar capacity of photoreactivation compared to wild-type. Over the pH range 6-9 no difference to wild-type. Over the pH range 9-11 the K281R mutant and the wild-type show more tolerance to the high pH (9.0-11.0), and at pH 11.0, 78.5% and 62.3% of the activity are retained, respectively Dunaliella salina

Organism

Organism UniProt Comment Textmining
Dunaliella salina
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(6-4) photoproduct (in DNA)
-
 Dunaliella salina 2 pyrimidine residues (in DNA)
-
 ?

Synonyms

Synonyms Comment Organism
(6-4) photolyase
-
 Dunaliella salina
Ds64PHR
-
 Dunaliella salina

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
 assay carried out at room temperature Dunaliella salina

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
 (6-4) photolyase in the alkaline treated algae cells exhibit much more efficient repair than that in the normally cultured algae cells Dunaliella salina

pH Range

pH Minimum pH Maximum Comment Organism
6 9 wild-type, K281G mutant and K281R mutant show no differences in this pH range. Highest activity is reached at pH 9.0 Dunaliella salina
9 11 in this pH range wild-type, K281G mutant and K281R mutant show different activities: The activity of K281G mutant declines sharply and less than 30% of the activity is retained at pH 11.0, whereas K281R mutant and the wild-type shows more tolerance to the high pH (9.0-11.0), and at pH 11.0, 78.5% and 62.3% of the activity are retained, respectively Dunaliella salina

Expression

Organism Comment Expression
Dunaliella salina mRNA level of Ds64PHR in 24 h dark-grown algae cells increases in response to the exposure of UV-C. The mRNA levels of Ds64PHR increases after alkaline treatment up