Literature summary for 4.1.99.13 extracted from

Yamamoto, J.; Hitomi, K.; Todo, T.; Iwai, S.
Synthesis of oligonucleotides containing the Dewar valence isomer of the (6-4) photoproduct and their application to (6-4) photolyase studies (2006), Nucleic Acids Symp. Ser., 50, 61-62.

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Organism

Organism	UniProt	Comment	Textmining
Xenopus laevis	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
(6-4) photoproduct (in DNA) = 2 pyrimidine residues (in DNA)	The overall repair reaction consists of two distinct steps, one of which is light-independent and the other one light-dependent. In the initial light-independent step, a 6-iminium ion is thought to be generated via proton transfer induced by two histidines highly conserved among the (6-4) photolyases.This intermediate spontaneously rearranges to form an oxetane intermediate by intramolecular nucleophilic attack. In the subsequent light-driven reaction, one electron is believed to be transferred from the fully reduced FAD cofactor (FADH-) to the oxetane intermediate thus forming a neutral FADH radical and an anionic oxetane radical, which spontaneously fractures. The excess electron is then back-transferred to the flavin radical restoring the fully reduced flavin cofactor and a pair of pyrimidine bases	Xenopus laevis	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(6-4) photoproduct (in DNA)	-	Xenopus laevis	2 pyrimidine residues (in DNA)	-	?
Dewar photoproduct	although the affinity of the enzyme for the Dewar photoproduct-containing duplex is similar to that for the (6-4) photoproduct containing substrate a repair rate could not be shown. These results indicate that the (6-4) photolyase binds the DNA containing the Dewar photoproduct and induces a structural change in DNA to some extent, suggesting a difference in the binding mode compared to the (6-4) photoproduct	Xenopus laevis	?	-	?

Synonyms

Synonyms	Comment	Organism
(6-4) photolyase	-	Xenopus laevis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Xenopus laevis

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Xenopus laevis

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Release 2023.1 (January 2023)