Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.99.12 extracted from

  • Xu, Z.; Lin, Z.; Wang, Z.; Chen, T.
    Improvement of the riboflavin production by engineering the precursor biosynthesis pathways in Escherichia coli (2015), Chin. J. Chem. Engin., 23, 1834-1839 .
No PubMed abstract available
Search for more literature for 4.1.99.12

Cloned(Commentary)

Cloned (Comment) Organism
gene ribB, overexpression in Escherichia coli wild-type strain RF01 Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information engineering of Escherichia coli for increased riboflavin production: overexpression of gene ribB to increase the flux from ribulose 5-phosphate to 3,4-dihydroxybutan-2-one 4-phosphate. Then ndk and gmk genes are overexpressed to enhance GTP supply. Subsequently, a R419L mutation is introduced into purA to reduce the flux from IMP to AMP. Co-overexpression of mutant purF and prs genes further increased riboflavin production. The final strain RF18S produces 387.6 mg riboflavin per liter with a yield of 44.8 mg riboflavin per gram glucose in shake-flask fermentations. The final titer and yield are 72.2% and 55.6%. Mutant strain and method evaluation, overview Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-ribulose 5-phosphate Escherichia coli
-
 formate + L-3,4-dihydroxybutan-2-one 4-phosphate
-
 ?
D-ribulose 5-phosphate Escherichia coli K-12 / MG1655
-
 formate + L-3,4-dihydroxybutan-2-one 4-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A7J0
-
-
Escherichia coli K-12 / MG1655 P0A7J0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribulose 5-phosphate
-
 Escherichia coli formate + L-3,4-dihydroxybutan-2-one 4-phosphate
-
 ?
D-ribulose 5-phosphate
-
 Escherichia coli K-12 / MG1655 formate + L-3,4-dihydroxybutan-2-one 4-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
DHBP synthase
-
 Escherichia coli
ribB
-
 Escherichia coli

General Information

General Information Comment Organism
physiological function 3,4-dihydroxy-2-butanone 4-phosphate (DHBP) and GTP are the precursors for riboflavin biosynthesis Escherichia coli