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Literature summary for 4.1.99.12 extracted from

  • Huembelin, M.; Griesser, V.; Keller, T.; Schurter, W.; Haiker, M.; Hohmann, H.P.; Ritz, H.; Richter, G.; Bacher, A.; Van Loon, A.P.G.M.
    GTP cyclohydrolase II and 3,4-dihydroxy-2-butanone 4-phosphate synthase are rate-limiting enzymes in riboflavin synthesis of an industrial Bacillus subtilis strain used for riboflavin production (1999), J. Indust. Microbiol. Biotechnol., 22, 1-7.
No PubMed abstract available

Application

Application Comment Organism
synthesis the enzyme is essential for industrial riboflavin production by Bacillus subtilis overproducing strains, overview Bacillus subtilis

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in strain 1012 by gene insertion in the sacB locus Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
additional information an additional single copy of the ribA gene introduced into the sacBlocus of the riboflavin production strain and constitutive expression from the medium strength vegI promoter leads to improved riboflavin titers and yields of riboflavin on glucose of up to 25%, strain VB2XL1, both enzymatic activities of RibA, the 3,4-dihydroxy-2-butanone 4-phosphate synthase activity located in the N-terminal half of the protein and the GTP cyclohydrolase II activity of the C-terminal domain, are necessary for the improved riboflavin productivity, method, overview Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-ribulose 5-phosphate Bacillus subtilis rate-limiting step in riboflavin biosynthesis formate + L-3,4-dihydroxy-2-butanone-4-phosphate
-
?
additional information Bacillus subtilis RibA is the rate limiting enzyme in an industrial riboflavin producing strain ?
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
gene ribA
-

Reaction

Reaction Comment Organism Reaction ID
D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate RibA is a bifunctional enzyme possessing 3,4-dihydroxy-2-butanone 4-phosphate synthase activity located in the N-terminal half of the protein and GTP cyclohydrolase II activity, EC 3.5.4.25, of the C-terminal domain, overview Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribulose 5-phosphate
-
Bacillus subtilis formate + L-3,4-dihydroxy-2-butanone-4-phosphate
-
?
D-ribulose 5-phosphate rate-limiting step in riboflavin biosynthesis Bacillus subtilis formate + L-3,4-dihydroxy-2-butanone-4-phosphate
-
?
additional information RibA is the rate limiting enzyme in an industrial riboflavin producing strain Bacillus subtilis ?
-
?
additional information RibA is a bifunctional enzyme possessing 3,4-dihydroxy-2-butanone 4-phosphate synthase activity located in the N-terminal half of the protein and GTP cyclohydrolase II activity, EC 3.5.4.25, of the C-terminal domain, overview Bacillus subtilis ?
-
?

Synonyms

Synonyms Comment Organism
More cf. EC 3.5.4.25 Bacillus subtilis
ribA
-
Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Bacillus subtilis