Application | Comment | Organism |
---|---|---|
synthesis | the enzyme is essential for industrial riboflavin production by Bacillus subtilis overproducing strains, overview | Bacillus subtilis |
Cloned (Comment) | Organism |
---|---|
overexpression in strain 1012 by gene insertion in the sacB locus | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | an additional single copy of the ribA gene introduced into the sacBlocus of the riboflavin production strain and constitutive expression from the medium strength vegI promoter leads to improved riboflavin titers and yields of riboflavin on glucose of up to 25%, strain VB2XL1, both enzymatic activities of RibA, the 3,4-dihydroxy-2-butanone 4-phosphate synthase activity located in the N-terminal half of the protein and the GTP cyclohydrolase II activity of the C-terminal domain, are necessary for the improved riboflavin productivity, method, overview | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose 5-phosphate | Bacillus subtilis | rate-limiting step in riboflavin biosynthesis | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? | |
additional information | Bacillus subtilis | RibA is the rate limiting enzyme in an industrial riboflavin producing strain | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
gene ribA | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate | RibA is a bifunctional enzyme possessing 3,4-dihydroxy-2-butanone 4-phosphate synthase activity located in the N-terminal half of the protein and GTP cyclohydrolase II activity, EC 3.5.4.25, of the C-terminal domain, overview | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose 5-phosphate | - |
Bacillus subtilis | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? | |
D-ribulose 5-phosphate | rate-limiting step in riboflavin biosynthesis | Bacillus subtilis | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? | |
additional information | RibA is the rate limiting enzyme in an industrial riboflavin producing strain | Bacillus subtilis | ? | - |
? | |
additional information | RibA is a bifunctional enzyme possessing 3,4-dihydroxy-2-butanone 4-phosphate synthase activity located in the N-terminal half of the protein and GTP cyclohydrolase II activity, EC 3.5.4.25, of the C-terminal domain, overview | Bacillus subtilis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | cf. EC 3.5.4.25 | Bacillus subtilis |
ribA | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Bacillus subtilis |