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Literature summary for 4.1.99.12 extracted from
- Yeast dihydroxybutanone phosphate synthase, an enzyme of the riboflavin biosynthetic pathway, has a second unrelated function in expression of mitochondrial respiration (2003), J. Biol. Chem., 278, 14698-14703.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene RIB3, expression of the wild-type gene restores the ability to respire in the aE280/U1 pet mutant A137T of Saccharomyces cerevisiae, which is partially deficient in cytochromes a, a3, and cytochrome b | Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A137T | naturally occuring Rib3 mutant which is partially deficient in cytochromes a, a3, and cytochrome b, the respiratory defect elicited by this mutation cannot be explained by a flavin insufficiency based on the following evidence: 1. growth of the aE280/U1 on respiratory substrates is not rescued by exogenous riboflavin, 2. the levels of flavin nucleotides are not significantly different in the mutant and wild type, phenotype, overview | Saccharomyces cerevisiae |
| additional information | construction of RIB3 disruption mutants, restoration by riboflavin of growth of a rib3 deletion mutant on glucose but not glycerol/ethanol, phenotype, overview | Saccharomyces cerevisiae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | mainly | Saccharomyces cerevisiae | 5829 | - |
| mitochondrion | in the intermembrane space, possibly associated with the outer membrane, minor enzyme portion | Saccharomyces cerevisiae | 5739 | - |
| additional information | subcellular localization analysis, overview | Saccharomyces cerevisiae | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22500 | - |
x * 28000, SDS-PAGE, x * 22500, about, sequence calculation | Saccharomyces cerevisiae |
| 28000 | - |
x * 28000, SDS-PAGE, x * 22500, about, sequence calculation | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-ribulose 5-phosphate | Saccharomyces cerevisiae | - |
formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? |  |
| additional information | Saccharomyces cerevisiae | the enzyme is involved in the riboflavin biosynthetic pathway, but has a second unrelated function in expression of mitochondrial respiration | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
gene RIB3, wild-type strain W303-1A | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-ribulose 5-phosphate | - |
Saccharomyces cerevisiae | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? | |
| additional information | the enzyme is involved in the riboflavin biosynthetic pathway, but has a second unrelated function in expression of mitochondrial respiration | Saccharomyces cerevisiae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 28000, SDS-PAGE, x * 22500, about, sequence calculation | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DHBP synthase | - |
Saccharomyces cerevisiae |
| dihydroxybutanone phosphate synthase | - |
Saccharomyces cerevisiae |
| Rib3 | - |
Saccharomyces cerevisiae |
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