Cloned (Comment) | Organism |
---|---|
gene ribB, DNA determination and analysis, overexpression in strain M15 | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
2 * 23000, SDS-PAGE, hydrodynamic analysis, NMR structure study, 2 * 23351, mass spectrometry | Escherichia coli |
23351 | - |
2 * 23000, SDS-PAGE, hydrodynamic analysis, NMR structure study, 2 * 23351, mass spectrometry | Escherichia coli |
44800 | - |
recombinant enzyme, analytical ultracentrifugation at 37°C | Escherichia coli |
46300 | - |
recombinant enzyme, hydrodynamic analysis, NMR structure study, analytical ultracentrifugation at 4°C, overview | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose 5-phosphate | Escherichia coli | the enzyme is involved in the pathway of riboflavin biosynthesis | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene ribB | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from strain M15 by anion exchange chromatography and gel filtration | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate | catalytic mechanism | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.152 | - |
purified recombinant enzyme | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose 5-phosphate | - |
Escherichia coli | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? | |
D-ribulose 5-phosphate | the enzyme is involved in the pathway of riboflavin biosynthesis | Escherichia coli | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 23000, SDS-PAGE, hydrodynamic analysis, NMR structure study, 2 * 23351, mass spectrometry | Escherichia coli |
More | hydrodynamic analysis, NMR structure study, isotope labeling, the homodimeric protein obeys strict C2 symmetry, overview | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
3,4-dihydroxy-2-butanone 4-phosphate synthase | - |
Escherichia coli |