Literature summary for 4.1.99.1 extracted from

Do, Q.T.; Nguyen, G.T.; Celis, V.; Phillips, R.S.
Inhibition of Escherichia coli tryptophan indole-lyase by tryptophan homologues (2014), Arch. Biochem. Biophys., 560, 20-26 .

Search for more literature for 4.1.99.1

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant enzyme expression in Escherichia coli strain JM101	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
(S)-4-(benzimidazol-1-yl)-2-aminobutanoic acid	-	Escherichia coli
2,3-dihydro-L-tryptophan	-	Escherichia coli
2-oxindolyl-L-alanine	-	Escherichia coli
L-bishomotryptophan	potent inhibitor, formation of an external aldimine. The compound is a selective inhibitor and is a potential lead for the development of antibacterials	Escherichia coli
L-homotryptophan	a moderate competitive inhibitor, formation of an external aldimine and quinonoid	Escherichia coli
L-tryptophan	weak substrate inhibition	Escherichia coli
additional information	design, synthesis, and evaluation of homotryptophan analogues as possible mechanism-based inhibitors for Escherichia coli tryptophan indole-lyase, overview. As a quinonoid structure is an intermediate in the reaction mechanism of TIL, homologation of the physiological substrate, L-Trp, might provide analogues resembling the transition state for beta-elimination, and potentially inhibit the enzyme. Kinetics show that formation of a quinonoid complex is not required for strong inhibition	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-tryptophan + H2O	Escherichia coli	-	indole + pyruvate + NH3	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0A853	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme from Escherichia coli strain JM101	Escherichia coli

Reaction

Reaction	Comment	Organism	Reaction ID
L-tryptophan + H2O = indole + pyruvate + NH3	proposed mechanism of tryptophan indole-lyase, overview	Escherichia coli	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-tryptophan + H2O	-	Escherichia coli	indole + pyruvate + NH3	-	?

Synonyms

Synonyms	Comment	Organism
TIL	-	Escherichia coli
tryptophan indole-lyase	-	Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Escherichia coli

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	dependent on	Escherichia coli

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
additional information	-	additional information	pre-steady-state inhibition kinetics, rapid-scanning stopped-flow experiments	Escherichia coli
0.002	-	2,3-dihydro-L-tryptophan	pH 8.0, 25°C, recombinant enzyme	Escherichia coli
0.0047	-	L-bishomotryptophan	pH 8.0, 25°C, recombinant enzyme	Escherichia coli
0.005	-	2-oxindolyl-L-alanine	pH 8.0, 25°C, recombinant enzyme	Escherichia coli
0.013	-	(S)-4-(benzimidazol-1-yl)-2-aminobutanoic acid	pH 8.0, 25°C, recombinant enzyme	Escherichia coli
0.067	-	L-homotryptophan	pH 8.0, 25°C, recombinant enzyme	Escherichia coli
0.2	-	L-tryptophan	pH 8.0, 25°C, recombinant enzyme	Escherichia coli

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Release 2023.1 (January 2023)