Cloned (Comment) | Organism |
---|---|
gene tna2 or tnaA2, the transcriptional start site within the tna2 promoter is preceded by the -24 and -12 consensus sequences specified by an alternative sigma factor, sigma54 (UniProt Q67SC0), phylogenetic analysis, quantitative real-time RT-PCR enzyme expression analysis, coculture-dependent expression of tryptophanase in Symbiobacterium thermophilum, overview | Symbiobacterium thermophilum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tryptophan + H2O | Symbiobacterium thermophilum | - |
indole + pyruvate + NH3 | - |
? | |
L-tryptophan + H2O | Symbiobacterium thermophilum T / IAM 14863 / JCM 14929 | - |
indole + pyruvate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Symbiobacterium thermophilum | P31015 | - |
- |
Symbiobacterium thermophilum T / IAM 14863 / JCM 14929 | P31015 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | tryptophanase-positive Symbiobacterium thermophilum is a free-living syntrophic bacterium that grows effectively in a coculture with Geobacillus stearothermophilus strain S (JCM 30104) | Symbiobacterium thermophilum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tryptophan + H2O | - |
Symbiobacterium thermophilum | indole + pyruvate + NH3 | - |
? | |
L-tryptophan + H2O | - |
Symbiobacterium thermophilum T / IAM 14863 / JCM 14929 | indole + pyruvate + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
tna2 | - |
Symbiobacterium thermophilum |
tnaA2 | - |
Symbiobacterium thermophilum |
tryptophanase 2 | - |
Symbiobacterium thermophilum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent on | Symbiobacterium thermophilum |
Organism | Comment | Expression |
---|---|---|
Symbiobacterium thermophilum | bicarbonate stimulated transcription of gene tna2. The transcription of some genes involved in amino acid metabolism is sigma54-dependent, and a bacterial enhancer-binding protein containing a PAS domain controls the transcription under the presence of high levels of bicarbonate, coculture-dependent expression of tryptophanase in Symbiobacterium thermophilum, overview | up |
General Information | Comment | Organism |
---|---|---|
additional information | tryptophanase-positive Symbiobacterium thermophilum is a free-living syntrophic bacterium that grows effectively in a coculture with Geobacillus stearothermophilus, indole accumulation occurs when bicarbonate is added to the medium. The transcription of some genes involved in amino acid metabolism is sigma54-dependent, and a bacterial enhancer-binding protein containing a PAS domain controls the transcription under the presence of high levels of bicarbonate. sigma54-Dependent expression of tryptophanase in Symbiobacterium thermophilum, overview | Symbiobacterium thermophilum |