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Literature summary for 4.1.99.1 extracted from

  • hang, Y.; Hong, G.
    Evidences of Hfq associates with tryptophanase and affects extracellular indole levels (2009), Acta Biochim. Biophys. Sin., 41, 709-717.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1732
-
L-Trp in the presence of Hfq protein, in 0.1 M potassium phosphate buffer (pH 7.8), at 37°C Escherichia coli
0.1901
-
L-Trp in the absence of Hfq protein, in 0.1 M potassium phosphate buffer (pH 7.8), at 37°C Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
tryptophanase subunit, SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
strains MG1655, MG1406, and MG1655H
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.508
-
cell extract from strain MG1655H, harvested at OD600 of 0.8, at 37°C Escherichia coli
0.747
-
cell extract from strain MG1655, harvested at OD600 of 0.4, at 37°C Escherichia coli
0.774
-
cell extract from strain MG1406, harvested at OD600 of 0.4, at 37°C Escherichia coli
0.808
-
cell extract from strain MG1655H, harvested at OD600 of 0.4, at 37°C Escherichia coli
0.818
-
cell extract from strain MG1655, harvested at OD600 of 0.8, at 37°C Escherichia coli
1.475
-
cell extract from strain MG1406, harvested at OD600 of 0.8, at 37°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Trp + H2O
-
Escherichia coli indole + pyruvate + NH4+
-
?

Synonyms

Synonyms Comment Organism
Trpase
-
Escherichia coli
tryptophan indole-lyase
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Escherichia coli

General Information

General Information Comment Organism
physiological function Hfq protein associates with tryptophanase under relatively higher extracellular indole levels, suggesting this is a feedback control of indole production Escherichia coli