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Literature summary for 4.1.99.1 extracted from

  • Kulikova, V.V.; Zakomirdina, L.N.; Dementieva, I.S.; Phillips, R.S.; Gollnick, P.D.; Demidkina, T.V.; Faleev, N.G.
    Tryptophanase from Proteus vulgaris: The conformational rearrangement in the active site, induced by the mutation of tyrosine 72 to phenylalanine, and its mechanistic consequences (2006), Biochim. Biophys. Acta, 1764, 750-757.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
Y72F mutation leads to a decrease in activity for L-tryptophan by 50000fold and to a considerable rearrangement of the active site. This rearrangement leads to an increase of room around the alpha -C atom of any bound amino acid, such that covalent binding of alpha -methyl-substituted amino acids becomes possible (which cannot be realized in wild-type Trpase) Proteus vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
L-Trp pH 7.8, 37°C, mutant enzyme Y72F Proteus vulgaris
0.15
-
S-benzylcysteine pH 7.8, 37°C, mutant enzyme Y72F Proteus vulgaris
0.18
-
S-methyl-L-Cys pH 7.8, 37°C, mutant enzyme Y72F Proteus vulgaris
0.18
-
S-o-nitrophenyl-L-Cys pH 7.8, 37°C, mutant enzyme Y72F Proteus vulgaris
0.81
-
S-ethyl-L-cysteine pH 7.8, 37°C, mutant enzyme Y72F Proteus vulgaris
4.64
-
L-Ser pH 7.8, 37°C, mutant enzyme Y72F Proteus vulgaris
6.15
-
beta-chloroalanine pH 7.8, 37°C, mutant enzyme Y72F Proteus vulgaris

Organism

Organism UniProt Comment Textmining
Proteus vulgaris
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-chloroalanine + H2O
-
Proteus vulgaris pyruvate + NH4+ + Cl-
-
?
L-Ser + H2O
-
Proteus vulgaris pyruvate + H2O
-
?
L-Trp + H2O
-
Proteus vulgaris indole + pyruvate + NH4+
-
?
S-benzylcysteine + H2O
-
Proteus vulgaris pyruvate + NH4+ + phenylmethanethiol
-
?
S-ethyl-L-cysteine + H2O
-
Proteus vulgaris pyruvate + NH4+ + ethanethiol
-
?
S-methyl-L-Cys + H2O
-
Proteus vulgaris pyruvate + NH4+ + methanethiol
-
?
S-o-nitrophenyl-L-Cys + H2O
-
Proteus vulgaris o-nitrothiophenol + pyruvate + NH3
-
?

Synonyms

Synonyms Comment Organism
Trpase
-
Proteus vulgaris

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00004
-
Trp pH 7.8, 37°C, mutant enzyme Y72F Proteus vulgaris
0.00046
-
S-benzylcysteine pH 7.8, 37°C, mutant enzyme Y72F Proteus vulgaris
0.0051
-
S-methyl-L-Cys pH 7.8, 37°C, mutant enzyme Y72F Proteus vulgaris
0.0303
-
L-Ser pH 7.8, 37°C, mutant enzyme Y72F Proteus vulgaris
0.035
-
S-ethyl-L-cysteine pH 7.8, 37°C, mutant enzyme Y72F Proteus vulgaris
0.0932
-
beta-chloroalanine pH 7.8, 37°C, mutant enzyme Y72F Proteus vulgaris
0.423
-
S-o-nitrophenyl-L-Cys pH 7.8, 37°C, mutant enzyme Y72F Proteus vulgaris