Protein Variants | Comment | Organism |
---|---|---|
Y72F | mutation leads to a decrease in activity for L-tryptophan by 50000fold and to a considerable rearrangement of the active site. This rearrangement leads to an increase of room around the alpha -C atom of any bound amino acid, such that covalent binding of alpha -methyl-substituted amino acids becomes possible (which cannot be realized in wild-type Trpase) | Proteus vulgaris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
L-Trp | pH 7.8, 37°C, mutant enzyme Y72F | Proteus vulgaris | |
0.15 | - |
S-benzylcysteine | pH 7.8, 37°C, mutant enzyme Y72F | Proteus vulgaris | |
0.18 | - |
S-methyl-L-Cys | pH 7.8, 37°C, mutant enzyme Y72F | Proteus vulgaris | |
0.18 | - |
S-o-nitrophenyl-L-Cys | pH 7.8, 37°C, mutant enzyme Y72F | Proteus vulgaris | |
0.81 | - |
S-ethyl-L-cysteine | pH 7.8, 37°C, mutant enzyme Y72F | Proteus vulgaris | |
4.64 | - |
L-Ser | pH 7.8, 37°C, mutant enzyme Y72F | Proteus vulgaris | |
6.15 | - |
beta-chloroalanine | pH 7.8, 37°C, mutant enzyme Y72F | Proteus vulgaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Proteus vulgaris | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-chloroalanine + H2O | - |
Proteus vulgaris | pyruvate + NH4+ + Cl- | - |
? | |
L-Ser + H2O | - |
Proteus vulgaris | pyruvate + H2O | - |
? | |
L-Trp + H2O | - |
Proteus vulgaris | indole + pyruvate + NH4+ | - |
? | |
S-benzylcysteine + H2O | - |
Proteus vulgaris | pyruvate + NH4+ + phenylmethanethiol | - |
? | |
S-ethyl-L-cysteine + H2O | - |
Proteus vulgaris | pyruvate + NH4+ + ethanethiol | - |
? | |
S-methyl-L-Cys + H2O | - |
Proteus vulgaris | pyruvate + NH4+ + methanethiol | - |
? | |
S-o-nitrophenyl-L-Cys + H2O | - |
Proteus vulgaris | o-nitrothiophenol + pyruvate + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Trpase | - |
Proteus vulgaris |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00004 | - |
Trp | pH 7.8, 37°C, mutant enzyme Y72F | Proteus vulgaris | |
0.00046 | - |
S-benzylcysteine | pH 7.8, 37°C, mutant enzyme Y72F | Proteus vulgaris | |
0.0051 | - |
S-methyl-L-Cys | pH 7.8, 37°C, mutant enzyme Y72F | Proteus vulgaris | |
0.0303 | - |
L-Ser | pH 7.8, 37°C, mutant enzyme Y72F | Proteus vulgaris | |
0.035 | - |
S-ethyl-L-cysteine | pH 7.8, 37°C, mutant enzyme Y72F | Proteus vulgaris | |
0.0932 | - |
beta-chloroalanine | pH 7.8, 37°C, mutant enzyme Y72F | Proteus vulgaris | |
0.423 | - |
S-o-nitrophenyl-L-Cys | pH 7.8, 37°C, mutant enzyme Y72F | Proteus vulgaris |