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Literature summary for 4.1.99.1 extracted from

  • Cowell, J.L.; Moser, K.; DeMoss, R.D.
    Tryptophanase from Aeromonas liquefaciens. Purification, molecular weight and some chemical, catalytic and immunochemical properties (1973), Biochim. Biophys. Acta, 315, 449-463.
No PubMed abstract available

General Stability

General Stability Organism
repeated slow freezing at -20°C and subsequent thawing at room temperature causes the enzyme to aggregate Aeromonas hydrophila

Inhibitors

Inhibitors Comment Organism Structure
Ala competitive Aeromonas hydrophila
Ala
-
Escherichia coli
anthranilic acid competitive Aeromonas hydrophila
Phe competitive Aeromonas hydrophila
Phe
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
L-Trp
-
Aeromonas hydrophila

Metals/Ions

Metals/Ions Comment Organism Structure
K+
-
Escherichia coli
K+ K+ or NH4+ required Aeromonas hydrophila
K+ K+ or NH4+ required Paenibacillus alvei
NH4+
-
Escherichia coli
NH4+ K+ or NH4+ required Aeromonas hydrophila
NH4+ K+ or NH4+ required Paenibacillus alvei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
216000
-
aggregation to form species with MW 432000 Da, 648000 Da, and 864000 Da, sucrose density gradient centrifugation, equilibrium sedimentation Aeromonas hydrophila

Organism

Organism UniProt Comment Textmining
Aeromonas hydrophila
-
-
-
Escherichia coli
-
-
-
Fusobacterium necrophorum subsp. funduliforme
-
-
-
Micrococcus aerogenes
-
-
-
Paenibacillus alvei
-
-
-
Paracolobactrum coliforme
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Aeromonas hydrophila

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13.3
-
-
Aeromonas hydrophila

Storage Stability

Storage Stability Organism
-20°C, 8-10 mg/ml in 0.1 M potassium phosphate buffer, pH 7.8, containing 0.1 mM pyridoxal phosphate and 1.0 mM dithiothreitol, stable for up to 1 year Aeromonas hydrophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Trp + H2O
-
Escherichia coli indole + pyruvate + NH4+
-
?
L-Trp + H2O
-
Micrococcus aerogenes indole + pyruvate + NH4+
-
?
L-Trp + H2O
-
Aeromonas hydrophila indole + pyruvate + NH4+
-
?
L-Trp + H2O
-
Paenibacillus alvei indole + pyruvate + NH4+
-
?
L-Trp + H2O
-
Fusobacterium necrophorum subsp. funduliforme indole + pyruvate + NH4+
-
?
L-Trp + H2O
-
Paracolobactrum coliforme indole + pyruvate + NH4+
-
?

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate contains 4 mol of pyridoxal 5'-phosphate per mol of enzyme Aeromonas hydrophila
pyridoxal 5'-phosphate dependent on Escherichia coli
pyridoxal 5'-phosphate dependent on Aeromonas hydrophila
pyridoxal 5'-phosphate dependent on Paenibacillus alvei
pyridoxal 5'-phosphate dependent on Fusobacterium necrophorum subsp. funduliforme
pyridoxal 5'-phosphate Km: 0.00209 mM Aeromonas hydrophila