General Stability | Organism |
---|---|
repeated slow freezing at -20°C and subsequent thawing at room temperature causes the enzyme to aggregate | Aeromonas hydrophila |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ala | competitive | Aeromonas hydrophila | |
Ala | - |
Escherichia coli | |
anthranilic acid | competitive | Aeromonas hydrophila | |
Phe | competitive | Aeromonas hydrophila | |
Phe | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
L-Trp | - |
Aeromonas hydrophila |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | - |
Escherichia coli | |
K+ | K+ or NH4+ required | Aeromonas hydrophila | |
K+ | K+ or NH4+ required | Paenibacillus alvei | |
NH4+ | - |
Escherichia coli | |
NH4+ | K+ or NH4+ required | Aeromonas hydrophila | |
NH4+ | K+ or NH4+ required | Paenibacillus alvei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
216000 | - |
aggregation to form species with MW 432000 Da, 648000 Da, and 864000 Da, sucrose density gradient centrifugation, equilibrium sedimentation | Aeromonas hydrophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeromonas hydrophila | - |
- |
- |
Escherichia coli | - |
- |
- |
Fusobacterium necrophorum subsp. funduliforme | - |
- |
- |
Micrococcus aerogenes | - |
- |
- |
Paenibacillus alvei | - |
- |
- |
Paracolobactrum coliforme | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Aeromonas hydrophila |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13.3 | - |
- |
Aeromonas hydrophila |
Storage Stability | Organism |
---|---|
-20°C, 8-10 mg/ml in 0.1 M potassium phosphate buffer, pH 7.8, containing 0.1 mM pyridoxal phosphate and 1.0 mM dithiothreitol, stable for up to 1 year | Aeromonas hydrophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Trp + H2O | - |
Escherichia coli | indole + pyruvate + NH4+ | - |
? | |
L-Trp + H2O | - |
Micrococcus aerogenes | indole + pyruvate + NH4+ | - |
? | |
L-Trp + H2O | - |
Aeromonas hydrophila | indole + pyruvate + NH4+ | - |
? | |
L-Trp + H2O | - |
Paenibacillus alvei | indole + pyruvate + NH4+ | - |
? | |
L-Trp + H2O | - |
Fusobacterium necrophorum subsp. funduliforme | indole + pyruvate + NH4+ | - |
? | |
L-Trp + H2O | - |
Paracolobactrum coliforme | indole + pyruvate + NH4+ | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | contains 4 mol of pyridoxal 5'-phosphate per mol of enzyme | Aeromonas hydrophila | |
pyridoxal 5'-phosphate | dependent on | Escherichia coli | |
pyridoxal 5'-phosphate | dependent on | Aeromonas hydrophila | |
pyridoxal 5'-phosphate | dependent on | Paenibacillus alvei | |
pyridoxal 5'-phosphate | dependent on | Fusobacterium necrophorum subsp. funduliforme | |
pyridoxal 5'-phosphate | Km: 0.00209 mM | Aeromonas hydrophila |