Cloned (Comment) | Organism |
---|---|
apo-ACP | Klebsiella pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
11760 | - |
apo-ACP-His, mass spectrometry | Escherichia coli |
11760 | - |
apo-ACP-His, mass spectrometry | Klebsiella pneumoniae |
12500 | - |
apo-ACP-His, SDS-PAGE | Escherichia coli |
12500 | - |
apo-ACP-His, SDS-PAGE | Klebsiella pneumoniae |
12650 | - |
holo-ACP-His, mass spectrometry | Escherichia coli |
12650 | - |
holo-ACP-His, mass spectrometry | Klebsiella pneumoniae |
15000 | - |
holo-ACP-His, SDS-PAGE | Escherichia coli |
15000 | - |
holo-ACP-His, SDS-PAGE | Klebsiella pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
citrate | Escherichia coli | - |
acetate + oxaloacetate | - |
? | |
citrate | Klebsiella pneumoniae | - |
acetate + oxaloacetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Klebsiella pneumoniae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
apo-ACP | Klebsiella pneumoniae |
apo-ACP, i.e. gamma-subunit | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8 | - |
- |
Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
citrate | - |
Escherichia coli | acetate + oxaloacetate | - |
? | |
citrate | - |
Klebsiella pneumoniae | acetate + oxaloacetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | alpha, beta, gamma | Klebsiella pneumoniae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Klebsiella pneumoniae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
2'-(5''-phosphoribosyl)-3'-dephospho-coenzyme A | attached to the gamma subunit, i.e. apo-ACP, via a phosphodiester linkage to serine-14, CitG and CitX catalyze the initial formation of the precursor 2'-(5''-triphosphoribosyl)-3'-dephospho-CoA and subsequent transfer to apo-citrate lyase with removal of diphosphate | Klebsiella pneumoniae | |
2'-(5''-phosphoribosyl)-3'-dephospho-coenzyme A | attached to the gamma subunit, i.e. apo-ACP, via phosphodiester linkage by CitX, resulting in the formation of holo-ACP | Escherichia coli |