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Literature summary for 4.1.3.6 extracted from

  • Antranikian, G.; Klinner, C.; Kuemmel, A.; Schwanitz, D.; Zimmermann, T.; Mayer, F.; Gottschalk, G.
    Purification of L-glutamate-dependent citrate lyase from Clostridium sphenoides and electron microscopic analysis of citrate lyase isolated from Rhodopseudomonas gelatinosa, Streptococcus diacetilactis and C. sphenoides (1982), Eur. J. Biochem., 126, 35-42.
    View publication on PubMed
Search for more literature for 4.1.3.6

Activating Compound

Activating Compound Comment Organism Structure
L-Glu activity is strictly dependent on presence of L-Glu Lacrimispora sphenoides

General Stability

General Stability Organism
only stable in presence of 150 mM L-Glu or 7 mM L-Glu plus glycerol, sucrose or bovine serum albumin Lacrimispora sphenoides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
 citrate
-
 Lacrimispora sphenoides

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ strict requirement for a divalent metal ion, Mg2+, Mn2+ or Co2+ Lacrimispora sphenoides
Mg2+
-
 Lactococcus lactis subsp. lactis
Mg2+ strict requirement for a divalent metal ion, Mg2+, Mn2+ or Co2+. Mg2+ is most effective Lacrimispora sphenoides
Mn2+
-
 Lactococcus lactis subsp. lactis
Mn2+ strict requirement for a divalent metal ion, Mg2+, Mn2+ or Co2+ Lacrimispora sphenoides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
515000
-
 gel filtration, sucrose density gradient centrifugation Lacrimispora sphenoides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
citrate Lacrimispora sphenoides regulated by concentration of intracellular L-Glu ?
-
 ?

Organism

Organism UniProt Comment Textmining
Lacrimispora sphenoides
-
-
-
Lactococcus lactis subsp. lactis
-
-
-
Rubrivivax gelatinosus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lacrimispora sphenoides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Lactococcus lactis subsp. lactis
additional information
-
-
 Lacrimispora sphenoides
additional information
-
-
 Rubrivivax gelatinosus

Storage Stability

Storage Stability Organism
-20°C, stable for 2 months Lacrimispora sphenoides
0°C, 30% glycerol, 40% sucrose or 10 mg per ml bovine serum albumin, 7 mM L-Glu, only some loss of activity after 5 min Lacrimispora sphenoides
4°C, stable for 4 days Lacrimispora sphenoides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
citrate
-
 Lactococcus lactis subsp. lactis acetate + oxaloacetate
-
 ?
citrate
-
 Lacrimispora sphenoides acetate + oxaloacetate
-
 ?
citrate
-
 Rubrivivax gelatinosus acetate + oxaloacetate
-
 ?
citrate regulated by concentration of intracellular L-Glu Lacrimispora sphenoides ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 half-life: 4 h Lacrimispora sphenoides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
 Lacrimispora sphenoides