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Literature summary for 4.1.3.45 extracted from

  • Andexer, J.N.; Kendrew, S.G.; Nur-e-Alam, M.; Lazos, O.; Foster, T.A.; Zimmermann, A.S.; Warneck, T.D.; Suthar, D.; Coates, N.J.; Koehn, F.E.; Skotnicki, J.S.; Carter, G.T.; Gregory, M.A.; Martin, C.J.; Moss, S.J.; Leadlay, P.F.; Wilkinson, B.
    Biosynthesis of the immunosuppressants FK506, FK520, and rapamycin involves a previously undescribed family of enzymes acting on chorismate (2011), Proc. Natl. Acad. Sci. USA, 108, 4776-4781.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli and in Streptomyces hygroscopicus BIOT-4010 cells Streptomyces hygroscopicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.53
-
chorismate in deuterated potassium phosphate buffer (20 mM, pH 7.0), at 20°C Streptomyces hygroscopicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chorismate Streptomyces hygroscopicus
-
3-hydroxybenzoate + pyruvate + H2O
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces hygroscopicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Streptomyces hygroscopicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chorismate
-
Streptomyces hygroscopicus 3-hydroxybenzoate + pyruvate + H2O
-
?

Synonyms

Synonyms Comment Organism
3-hydroxybenzoate synthase
-
Streptomyces hygroscopicus
3HBA synthase
-
Streptomyces hygroscopicus
hyg5
-
Streptomyces hygroscopicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
22.4
-
chorismate in deuterated potassium phosphate buffer (20 mM, pH 7.0), at 20°C Streptomyces hygroscopicus