Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
overexpressed in pKK223.3 expression vector | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | gene inactivation | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.6 | - |
(4S)-4-hydroxy-2-oxoglutarate | pH 8.4, 25°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22000 | - |
3 * 22284, calculated, 3 * 22000, SDS-PAGE | Escherichia coli |
22284 | - |
3 * 22284, calculated, 3 * 22000, SDS-PAGE | Escherichia coli |
62500 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli | - |
overexpressed after cloning into pKK223.3 expression vector | - |
Escherichia coli | P0A955 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
80fold increase in specific activity after overexpression in pKK223.3 | Escherichia coli |
45 | - |
pH 8.4, 25°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(4S)-4-hydroxy-2-oxoglutarate | - |
Escherichia coli | pyruvate + glyoxylate | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 22284, calculated, 3 * 22000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
2-keto-4-hydroxybutyrate aldolase | ambiguous | Escherichia coli |
2-keto-4-hydroxyglutarate aldolase | ambiguous | Escherichia coli |
2-keto-4-hydroxyglutaric aldolase | ambiguous | Escherichia coli |
2-oxo-4-hydroxyglutarate aldolase | ambiguous | Escherichia coli |
2-oxo-4-hydroxyglutaric aldolase | ambiguous | Escherichia coli |
4-hydroxy-2-ketoglutarate aldolase | ambiguous | Escherichia coli |
4-hydroxy-2-ketoglutaric aldolase | ambiguous | Escherichia coli |
4-hydroxy-2-oxoglutarate glyoxylate-lyase | ambiguous | Escherichia coli |
eda | - |
Escherichia coli |
HGA | - |
Escherichia coli |
hydroxyketoglutarate aldolase | ambiguous | Escherichia coli |
Hydroxyketoglutaric aldolase | ambiguous | Escherichia coli |
KHG/KDPG aldolase | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | - |
- |
Escherichia coli |